2 is correct
5 is incorrect
8-12 are correct
15 and 18 are incorrect
19-20, 22 are correct.

Mind you I'm not sure we ever saw Sister Voodoo's parents so she could have been created in an alien laboratory or something.

Andy E. Nystrom wrote:

The latest quiz is a Marvel grab bag. No theme beyond it being Marvel Multiverse related.

1. Which cartoon character appeared first appeared in a comic book tie in before the series began?
3. What was the catch-phrase of the Scourge parody in What The--?!?
4.  One ad featured which Marvel super-villain taunting which Marfel superhero as the latter played their own video game?
5. Who starred in the final issue of Questprobe?
6. Who teamed with Spider-Man in Marvel Team-Up #150?
7. Who teamed with the Thing in Marvel Two-in-One #100?
18. Which Sub-Mariner foe later joined Heroes for Hire?
21. Which Avenger shared a code name with one of the Imperial Guard?
 

1. Video-Man?
3. "Dinner is Served!"
4. Green Goblin, Spider-Man (I assumed you mean Marvel instead of Marfel)
5. the Thing and the Human Torch (final issue of the series); the X-Men (story for #4 published in Marvel Fanfare a few years later)
6. the X-Men
7. Ben Grimm
18. Orca
21. Quasar

Andy E. Nystrom wrote:

1 is incorrect but you're on the right track. The rest are correct. Ben was from a world where the 616 Thing had previously cured him. Come to think of it, he's someone who probably deserves a profile.

1. Firestar

Yes, from Marvel Two-in-One #50

Andy E. Nystrom wrote:

1 is correct and I should have actually said two characters. Who else first appeared in any incarnation in Spider-Man and His Amazing Friends #1?

Ms. Lion. I initially guessed Video-Man because I thought Firestar would be too obvious,

16 Punfisher
17 Remnant

Calling a day on the following:
13. In a Speedball story in Marvel Super-Heroes, two guys killed each other over which toy gun?
14. What is the first name of Forbush Man's aunt?
15. Who wrote Spider-Man's second team-up with Superman?

Incorrect. It's a fictional toy, not an actual toy brand.

The latest theme is issue #1. First series unless otherwise noted

1. Which character from Action Comics #1 later became Mister America?
2. Issue #1 of which series had a cover showing characters looking at possible recruits?
3. Kymellians were introduced in #1 of which series?
4. Which villain was introduced in Amazing Spider-Man #1?
5. Who was the villain in Howard the Duck #1, who guest starred in that issue, and which other major character first appeared there?
6. What was the first appearance of the MAX Punisher?
7. What was the first appearance of Archie's second Shield?
8. What did She-Hulk threaten to do on the cover of Sensational She-Hulk #1?
9. Who was the villain in Green Lantern #1 (second series/first with Hal)?
10. Who died in their own Annual #1 and has never returned from the dead?
11. Where did Conan encounter Ymir's daughter?
12. Where did three future charter members of the Defenders first team up?
13. Where was the first time that that three non-charter future Defenders were seen teaming up?
14. What was the indicia title of Not Brand Echh #1?
15, What was Slam Bradley's first appearance?
16. What was the first comic published by National Periodical Publications?
17. What was the first comic published by Timely Comics, and what was the earlier first appearance of one of the future characters (and which one)?
18. Who was the villain seen at the end of The Flash #1 (second series but first to have a #1)?
19. Where did Impulse become the Flash?
20. Where did Psimon join an ad hoc hero team?
21. Marvel published this one shot of a network's cartoon line-up
22. How does the sexual assault of Starlight in The Boys #1 differ from the TV version?
23. What was the first Human Torch series called in #1?
24. 711 first appeared where?
25. Where did comics' first patriotic superhero first appear (and which one and which publisher as they were known as at the time)?

 

2 and 21 are incorrect. The rest are correct (hadn't known about American Ace; Sub-Mariner of course being far better known).
 

21. Disney Primer?

10 Marc Hazzard, Merc
15 Detective Comics#1
17 could also be Ka-Zar, who had appeared previously in the pulp novel Ka-Zar, King of Fang and Claw
22 In the TV show she's forced to give oral sex to the Deep, in the comic every single male member of the team is involved in the assault.
 

More #1 issue questions (just Marvel this time):

26. “The Death of Captain Marvel" is otherwise known as...?
27. Angel was not the star of this series, but he was the most prominent character on the cover of the first issue.
28. Aside from the title character, the cover of this series’s first issue also featured Spider-Man, Iron Man, Nightcrawler, and the Enchantress.
29. Doctor Druid first appeared in what issue going by what name?
30. Due to a retcon, Misty Knight is now considered to have first appeared in what issue?
31. Of the original five Avengers, who was the only one to have debuted in a #1 issue?
32. Savage Tales Vol. 2 #1 (1985) included a story that was later spun off into what series?
33. This is commonly considered the best-selling issue of all time.
34. This issue featured the first Marvel appearances of five Golden Age characters who were now in the public domain.
35. What issue proclaimed that it was "Printed in real KISS blood?
36. What literary character made his comics debut in Monsters Unleashed #1?
37. What miniseries’s first issue was actually numbered #1.1?
38. What series’s #1 issue was actually the twelfth  issue of that series to be published?
39. Who was featured on the cover of Marvel Age #1?
40. Who were the four featured characters in the first issue of Marvel Comics Presents?

all correct

29. correct
33. nope
35. Yes, Marvel Comics Super Special #1.
37. Almost, but you made two small errors.
38. One down, three to go.

Andy E. Nystrom wrote:

33. X-Men #1 (second series) then?

 

Yep