343 Martha Wayne, Batman Begins

349 Asabi, Lois and Clark

353 Phillips, Watchmen TV

372 Jane Crawford, Watchmen TV

Latest answers correct; list updated. Jane Crawford is worth 20 bonus shots, bringing Loki's total to 70.

364 Crispus Allen, Justice League

388 Arnold Flass, Batman Begins

393 Darkseid, Smallville

406 Garth / Aqualad, Titans

Cleaned up clues:
339. Original TV show character; 10s; member of black ops team
344. Original TV show character; 80s; professional singer
345. Original TV show character; 90s; aunt by adoption of major character
355. From a TV show; based on comics (arguably indirectly); 10s; Outsiders; successor to another villain filling same role in group
358. Original TV pilot character; 90s; reporter seen in just one scene
359. Original TV show character but named after a real person; 10s; news anchor
360. Original TV show character; 20s; janitor
366. From a commercial; based on comics (kind of); 80s; Action Comics; appears with another actor known for playing same character
369. From a TV show; based on comics; 10s; Outsiders; can communicate with technology
375. From a TV show; based on comics; 10s; Batman; seen only in portrait
377. From a TV show; based on comics; 20s; Batman; co-owner of restaurant
378. Original TV show character; 10s; identity usurped by another character
383. Original TV show character; 20s; enforcer for main villain
385. From a TV show; based on real person (at least in part); 10s; judge
394. Original TV show character; 00s; executive who briefly took charge of company when boss went missing
397. Original TV show character; 10s; son of super-villain
399. Original TV show character; 80s; robbed banks and jewelry stores
400. Original TV show character; 80s; archaeologist working for museum
407. From a movie; based on comics; 10s; Detective Comics; son of a billionnaire couple
408. From a TV show; based on earlier character same continuity; 20s; son of a vigilante
409. From a movie; based on comics; 90s; Steel; arms dealer
411. Original TV show character; 10s; telepathic meta-human therapist
413. Original TV show character; 00s; design engineer woorking on new train
414. From a TV show; based on comics; 20s; Brightest Day; impersonated a superhero
415. From a TV show; based on comics; 10s; New Teen Titans; assassin
417. Original TV show character; 20s; works for weapons manufacturer
418. From a TV show; based on comics; 10s; Outsiders; counterpart of cast character
420. Original TV show character; 00s; ex-lover of main character
424. Original movie character; 10s; wife of crime family leader
425. From a TV show; based on comics; 00s; Detective Comics; street cop
426. Original TV show character; 00s; owned a bar and safe house
427. From a movie; name only based on religion; 00s; Christianity; half-breed demon
429. Original movie character; 20s; incumbent mayor
432. From a TV show; based on comics; 10s; Batman; corrupt police detective
433. From a TV show; based on real person (at least in part); 10s; judge
437. Original TV show character; 00s; targeted due to actions by his brother
439. From a TV show; has elements of at least two comics character using same alias; 10s; Shadowpact and Batman: The Dark Knight; hypnotized to leave clues about the whereabouts of a villain's victims
440. Original TV show character; 80s; former partner of the main villain of the episode
443. From a TV show; based on comics; 10s; Detective Comics; seen only in fake reality
444. From a TV show; based on comics; 10s; Hellblazer; ex-wife of cast character
445. From a movie; somewhat based on comics; 10s; Detective Comics; made a bomb threat
446. From a TV show; based on comics; 10s; Batman; police commissioner
447. Original TV show character; 80s; has same name as a James Bond villain
450. Original TV show character; 20s; wife of key ally of main character
451. Original TV show character; 20s; doublecrossed main character
452. From a movie; based on comics; 00s; Watchmen; seen briefly in flashback
453. Original movie character; 00s; attended a dinner date
455. From a TV show; based on comics; 10s; Batman; I think this version only appeared in news broadcast
456. Original TV show character; 10s; law enforcement officer of sorts
458. Original TV show character; 10s; mother of superhero
459. Original TV show character; 90s; bar owner/criminal
462. From a TV show; based on comics; 10s; Swamp Thing; biologist
465. From a movie; based on comics; 00s; Batman; wealthy surgeon
466. From a TV show; based on comics; 00s; All-American Comics; super-villain on life support
467. From a TV show; based on comics; 80s; Action Comics; soldier turned farmer
468. Original TV show character; 80s; federal agent
469. Original TV show character; 20s; possessed by alien
474. Original TV show character; 70s; international terrorist
475. From a TV show; based on comics (comics version's first name unrevealed); 10s; Green Arrow: Year One; criminal who abused young girl
476. From a TV show; based on comics; 80s; Action Comics; farmer
478. From a TV show; based on comics (original comics version had different first name); 00s; Superman's Girl Friend, Lois Lane; mother of major villain
480. From a TV show; based on comics (comics version's first name unrevealed and this version gender swapped); 10s; Detective Comics; mercenary with poison-tipped blades
482. Original TV show character; 10s; high school dropout
485. From a TV show; based on comics; 10s; Outsiders; part of a family of villains
486. From a TV show; based on comics; 10s; Green Lantern; criminal CFO of a corporation
487. Original movie character; 80s; gas station attendant
488. From a movie; based on comics; 10s; Doctor Fate; psychiatrist
489. From a movie; code name based on comics; 90s; Batman; information sparse on this version but presumably a villainess
490. From a TV show; based on comics; 00s; Justice League; telepath
491. From a TV show; based on comics; 10s; Hellblazer; possessed people with enormous hunger
493. From a movie; based on TV show (TV version has different first name); 10s; Batman: The Animated Series' construction company owner
495. Original movie character; 80s; elementary school student
496. From a TV show; based on comics; 20s; Action Comics; can pass through objects
497. From a movie; based on comics; 80s; Swamp Thing; sister of main character
501. From a TV show; based on comics; 10s; Batman: The Long Halloween; criminal second-in-command
504. Original TV show character; 80s; bank robber
505. From a TV show; based on comics; 10s; Action Comics; presumed farmer
506. Original movie character; 10s; childhood bully of main character
509. Original TV show character; 10s; fallen angel
510. From a TV show; based on radio show; 90s; The Adventures of Superman; from a skit
512. From a TV show; based on earlier character same continuity; 20s; merged with counterpart for a time
513. Original TV show character; 20s; white supremacist
514. Original TV show character; 10s; mother of a villain
518. Original TV movie character; 70s; worked for main villain
519. Original TV show character; 00s; could create psychic illusions
520. From a TV show; based on comics; 10s; Showcase; metahuman physician
522. From a TV show; based on comics; 10s; Arkham Asylum: Living Hell; circus strongman
524. From a TV show; based on comics; 10s; Detective Comics; seen with another character this collage who was already identified
526. From a TV show; based on comics; 10s; Detective Comics; shape-shifter
527. Original TV show character; 70s; dropped out of school to become a mechanic
528. Original movie character; 10s; security guard until injured
530. From a TV show; based on comics; 10s; Tales of the Teen Titans; heroic son of a villain
531. Original movie character; 10s; Mayan shaman
532. Original TV show character; 10s; prison warden
537. Original movie character; 00s; board member
538. Original TV show character; 10s; circus acrobat
539. From a TV show; based on comics; 00s; Green Arrow; martial artist/archer
540. From a movie; based on comics; 10s; Joker; worked for major villain
541. From a TV show; based on religion/myths; 90s; probably impossible to trace to a specific source; personification of an abstract concept
542. Original TV show character; 00s; clone of major character's brother
543. Original TV show character; 00s; killed by wraith resembling main character
546. From a TV show; based on comics with slightly different surname, though named on radio show 70s; Action Comics, named in Adventures of Superman; seen in alternate reality Nazi Germany
549. From a TV show; shares code name with comics character but that's probably coincidental; 70s; musician who can control minds
551. Original TV show character; 10s; mother of recurring villains
557. Original TV show character; 90s; one of the main characters once tried to steal his food
558. From a TV show; based on comics; 10s; Detective Comics; part of special ops team of cops
560. From a webseries (I've been trying to avoid those but this one slipped in); based on comics; 10s; All Star Comics; in some sort of music competition
561. Original movie character; 80s; helped villain try to stave off effects of aging
563. From a commercial; based on comics; 80s; Detective Comics; did a shopping trip in costume
564. From a TV show; based on comics; 10s; tasked his three child brides to find him a fourth
565. From a TV show; based on comics; 00s; legal father of major character
567. From a commercial; based on comics; 90s; Detective Comics; seen in live show ad
568. Original TV show character; 00s; head of cyber-security firm
569. Original movie character; 00s; mentor to main character
570. Original TV show character; 00s; deputy turned sheriff
571. Original movie character; 10s; food bar cook
572. Original movie character; 90s; part of circus themed criminal gang
578. Original TV show character; 10s; Russian leader of corrupt organization
579. Original movie character, though at least one comics character has same name; 00s; can resurrect people
581. From a TV show; based on comics (gender swapped so slightly different first name); 10s;  Tales of the New Teen Titans; war veteran
583. From a TV show; dressed as character based on comics; 80s; Detective Comics; started to have a "romantic" encounter with another such character
585. Original TV show character; 00s; attempted a killing on a flight
586. Original TV show character; 70s; wanted to stop a horse from being put down
587. Original TV show character, though at least one comics character has same name; 20s; bounty hunter
590. Original TV show character; 10s; ally of main character
591. From a TV show; based on comics; 90s; Action Comics; older counterpart of main character
592. Original TV show character; 10s; wife of super-villain
594. From a TV show; based on earlier character same continuity and surname nod to earlier comics character who isn't a counterpart; 20s; Superboy; editor-in-chief
596. From a TV show; based on comics; 10s; Catwoman; police detective
598. From a TV show; based on comics; 00s; Superman; police detective
602. Original TV show character; 10s; possessed people
606. From a TV show; based on comics; 10s; Outsiders; part of a family of villains
607. Original TV show character; 70s; hypnotist
610. Original TV show character; 10s; helped defend town from invaders from another country
611. Original TV show character; 10s; legal guardian of cast character
612. From a TV show; based on comics; 10s; New Teen Titans; can transmute matter
616. From a TV show; based on comics (slightly different first name); 10s; Justice Society of America; counterpart of cast character
620. Original movie character; 00s; police detective in special unit
621. From a TV show; based on earlier character same continuity; 10s; counterpart of recurring villain
622. Original TV show character; 00s; reporter for newspaper
624. From a movie; based on real person; 00s; astronaut in movie and in real life
625. Original TV show character; 10s; metahuman with unrevealed powers
626. Original TV movie character; 70s; no info online about character and it's been decades since I saw the movie; I think from the image I cropped this from she's a criminal
627. From a TV show; based on comics (though likely different real name); 10s; Detective Comics; depicted as a family member
629. From a TV show; based on comics (different real name and slightly different code name); 10s; Green Lantern; various powers including strength and self-resurrection
631. From a TV show; based on radio show; 90s;     The Adventures of Superman; presumed editor
633. Original TV show character; 90s; minion of major villain
634. From a TV show; dressed as character based on comics; 80s; All Star Comics; started to have a "romantic" encounter with another such character
635. From a movie; based on comics; 10s; Batman/Huntress: Cry for Blood; brother of future vigilante
638. Original TV show character; 10s; multiple clones all sharing same name, though usually only one active at a time
639. From a TV show; based on comics; 20s; Wonder Woman; warrior and mentor
641. Original TV show character; 10s; general counsel for engineering firm
643. From a movie; code name based on comics; 90s; Detective Comics; robber
647. Original TV show character; 80s; dated main character briefly
649. Original TV show character; 80s; sister-in-law of main character
650. From a TV show; based on comics; 10s; JSA; cousin of cast character
651. From a TV show; based on comics; 10s; Hawk and Dove; abused by husband
652. Original movie character; 10s; co-worker of main character
653. Original TV show character; 20s; lawyer to main villain
654. From a TV show; based on comics; 20s; discovered she's from another world
657. Original movie character; 10s; secretary for main villain
660. From a TV show; based on comics; 90s; Showcase or Flash, depending on which version this is supposed to be; from skit
661. From a movie; based on comics; 00s; Richard Dragon, Kung-Fu Fighter;  thief turned assassin
662. From a TV show; based on comics; 10s; Action Comics; from skit
664. From a movie; code name based on comics; 70s; Detective Comics; son of another hero
665. Original TV show character though name alludes to real person; 20s; major villain
670. Original TV show character; 10s; law enforcement officer of sorts
671. From a movie; based on comics; 00s; Action Comics; reporter
673. From a TV show; based on comics; 20s; Action Comics; counterpart of major character
674. Original TV show character; 10s; worked for rebel organization

567 Batman, DC Comics Superheroes Live show (1996)

538 Alphonse Grayson, Gotham

542 Grant Gabriel, Smallville

489 Catwoman, Alyas Batman en Robin

662 Superman, Conan's San Diego Comic Con skit "Batman wants to join the Marvel Universe"