396 Rosenberg, Daredevil TV

252 Mary, Runaways

131 Thembi Wallace, Luke Cage

Cleaned up clues:

28. Original TV show character; 10s; former member of terrorist group; appeared in the third season of a series
35. Original TV show character; 10s; student at a special academy who attended lecture by major characters
56. From a commercial; based on comics; 00s; Captain America Comics; seen in underground base accessed from outside a building
84. Original TV show character; 10s; pilot who crash landed a special jet
144. Original TV show character; 10s; tech company employee; gave presentation
145. From a commercial; based on comics; 00s; Tales of Suspense; seen in underground base accessed from outside a building
146. Original TV show character; 10s; helped try to capture major character; member of some sort of clan
152. From a commercial; based on comics; 00s; Incredible Hulk; attempted to play volleyball but damaged the ball
159. Original TV show character; 10s; replaced by robotic being; some sort of agent
167. Original TV show character; 10s; helped army veteran in attempt to kill major villain;
178. From a commercial; based on comics; 00s; Fantastic Four; unlike other versions of this character, this version seems non-sentient; "assisted" in team's fight with arch-foe
187. From a commercial; based on comics; 00s; X-Men; seen in underground base accessed from outside a building
191. Original TV show character; 10s; police officer; tried unsuccessfully to prevent a murder and a kidnapping
192. Original TV show character; 10s; assigned the task of protecting super-powered person but that person turned on him and killed him
193. Original TV show character; 10s; police officer; investigated whether a major character had been murdered
204. Original TV show character; 10s; executive producer who eventually cast major character as a show's main character
235. Original TV show character; 10s; police officer
275. Original TV show character; 10s; police officer
281. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s; replaced major character's father
292. From a commercial; based on mythology; 00s; norse mythology; his choice of weapon is key to identifying this version of the character
300. Original TV show character; 10s; police officer
306. From a commercial; based on mythology; 00s; norse mythology; among heroes who considered their latest mission a wasted trip
310. Original TV show character; 10s; helped ambush major character
312. Original TV show character; 10s; police officer
320. Original TV show character; 10s; brother of major character
329. Original TV show character; 10s; non-US federal agent
330. Original TV show character; 10s; gang member
341. Original TV show character; 10s; police detective
357. Original TV show character; 10s; police officer
374. From a commercial; based on comics; 00s; Incredible Hulk; among heroes who considered their latest mission a wasted trip
383. Original TV show character; 10s; co-worker of main character
409. Original TV show character; 10s; enforcer
410. Original TV show character; 10s; corrupt police officer

Updated tally:
Agents of SHIELD    8
Jessica Jones    5
Daredevil TV    4
[non-soft drink beverage commercial]    3
Iron Fist    2
Luke Cage    2
[finance-related commercial]    2
Agent Carter    1
[fast food related commercial]    1
Cloak & Dagger    1
[soft drink commercial]    1
Runaways    1
[toy commercial]    1

 

35 Callie Hannigan, Agents of SHIELD

193 Cooper, Jessica Jones

300 Lombardo, Jessica Jones

235 Preston, Daredevil TV

357 A. Kennedy, Agents of SHIELD

281 John Mackenzie Chronicon, Agents of SHIELD

409 Kevin Singleton, Iron Fist

Those are correct; list updated.

56 Captain America, 145 Hawkeye, 187 Scarlet Witch, Marvel Superheroes Got Milk commercial

144 Mita Nansari, Runaways

Latest answers correct; list updated. I'll add in the missing clue shortly.