627. Gabby Talbott, Ultimate

I'll update the list in a moment (all correct). Yes, 394 is the doorman

Is 394 also a dooman in the comics?

455. Warren Worthington, Jr., Yost

570. William Drake, Yost

Removing the dead clues:

317. From a movie; based on comics; comics version more or less a hero. Comics debut: Strange Tales; Strange Tales debut was well after #100; 00s
322. From a TV show; based on comics; comics debut: X-Men; 00s
343. Original TV character; animated creators presumed Ralph Bakshi, Lin Carter, Fred Halliday, Ira Turek; from Spider-Man 1967
351. Original TV character; in universe possibly created by Nova (Richard Rider); 10s
352. From a TV show; based on comics; human (altered). Assuming show follows comics, he was altered by taking a formula of his own creation; 10s
355. From a TV show; based on comics; non-artificial being transformed by the Terrigenesis process; 10s
360. From a TV show; based on comics; comics debut: New Mutants; 00s
370. Original TV character but named after a real person. The real person with his name is usually a comic book writer, sometimes comics book artist; 10s
372. From a TV show; based on comics; 00s
373. From a TV show; based on comics; 10s
377. From a TV show; based on comics; 10s
390. From a movie; based on comics; 00s
394. From a TV show; based on comics; 70s or 80s; doorman in show and comics
396. From a TV show; based on comics; human (mutant); 00s
399. From a movie; based on comics; 10s
403. From a TV show; based on comics; 00s
406. Original TV character; 10s
409. From a TV show; based on comics; comics version a villain; 10s
416. From a TV show; based on comics; 00s
418. From a movie; based on comics; 00s
422. From a TV show; based on comics; 00s
423. From a TV show; based on comics; 00s
425. Original TV character; 70s or 80s
427. Original TV character; 10s
431. From a TV show; based on comics; 00s
443. From a TV show; based on comics; 00s
444. From a movie; based on comics; 10s
448. From a TV show; based on comics; 00s
451. Original TV character; 90s
452. Original TV character; 90s
458. From a movie; based on comics; 10s
460. Original TV character; 10s
471. Original TV character, subsequently used in movies; 90s
472. From a TV show; based on comics; 90s
477. Original movie character; 00s
484. From a TV show; based on comics; 00s
489. From a TV show; based on comics; 10s
493. Original TV character; 10s
494. Original TV character; 10s
502. Original TV character; 10s
507. From a movie; based on comics; 10s
510. From a TV show; based on comics; 90s
513. From a TV show; based on comics; 90s
515. From a TV show; based on comics; 00s
516. From a TV show; based on comics; 00s
517. From a TV show; based on comics; 00s
519. From a TV show; based on comics; 10s
520. From a TV show; based on comics; 90s
522. From a TV show; based on earlier show; 10s
528. From a TV show; based on comics; 00s
530. From a TV show; based on comics; 00s
532. Original TV character; 90s
533. From a TV show; based on comics; 10s
539. Original TV character; 00s
540. From a TV show; based on comics; 10s
541. Original TV character; 00s
551. From a TV show; based on comics; 10s
553. Original TV character but named after a real person. The real person with his name is a comic book writer; 90s
554. From a movie; based on comics; 00s
555. From a TV show; based on Asgardian mythology; 10s
560. From a TV show; based on comics; 90s
561. From a TV show; based on comics; 00s
562. Original TV characters; 10s
571. From a TV show; based on comics; 00s
572. Original TV character; 60s
574. From a TV show; based on comics; 00s
579. From a TV show; based on comics; 10s
580. From a TV show; based on comics; 90s
581. From a TV show; based on comics; 70s or 80s
584. From a TV show; based on comics; 90s
588. Original TV character; 90s
600. From a TV show; based on comics; 10s
602. From a TV show; based on comics; 90s
610. Original TV character; 10s
611. Original TV character; 90s
612. Original TV character; 10s
617. From a TV show; based on comics; 90s
622. Original TV character; 10s
624. From a TV show; based on comics; 10s
626. From a TV show; based on comics; 70s or 80s
633. Original TV character; 10s
637. From a TV show; based on comics; 10s
638. Original TV character; 10s

Revised I spy clues:
I spy...

A character seated in an arena
A character in the education field who is not an educator in the comics
The mother of a character found in part 1 of this 3-parter.
A character who scolded Spider-Man both in the show and in comics (not Jameson)
At least one more parent of an X-Men
Someone who was transformed into a living portal
At least five characters from other Earths in their shows or movies.

 

484 is correct. List updated. This time I did a formal count instead of eyeballing the list and there are three more x-parents to be found this collage.

571 is correct. List updated.

Time for character specific clues (first 40):
317. From a movie; based on comics; comics version more or less a hero. Comics debut: Strange Tales; Strange Tales debut was well after #100; 00s; extra-terrestrial resembling fantasy creature
322. From a TV show; based on comics; comics debut: X-Men; 00s; nickname can refer to a vegetable
343. Original TV character; animated creators presumed Ralph Bakshi, Lin Carter, Fred Halliday, Ira Turek; from Spider-Man 1967; part of her name is a type of currency
351. Original TV character; in universe possibly created by Nova (Richard Rider); 10s; messenger droid
352. From a TV show; based on comics; human (altered). Assuming show follows comics, he was altered by taking a formula of his own creation; 10s; part of name describes a type of motion
355. From a TV show; based on comics; non-artificial being transformed by the Terrigenesis process; 10s; was transformed into a living portal
360. From a TV show; based on comics; comics debut: New Mutants; 00s; name refers to a type of avalanche (but not based on Avalanche)
370. Original TV character but named after a real person. The real person with his name is usually a comic book writer, sometimes comics book artist; 10s; real person wrote a version of the comic that show was based on, with some controversial additions
372. From a TV show; based on comics; 00s; comics version has worn armour
373. From a TV show; based on comics; 10s; Asgardian, and name similar but not identical to someone from Norse mythology
377. From a TV show; based on comics; 10s; mother of a superhero
390. From a movie; based on comics; 00s; girlfriend of a superhero
394. From a TV show; based on comics; 70s or 80s; doorman in show and comics; a hero or team lives in the building he's a doorman for
396. From a TV show; based on comics; human (mutant); 00s; future version of a character who in the comics is a kid
399. From a movie; based on comics; 10s; sister of a superhero
403. From a TV show; based on comics; 00s; assistant to a CEO who's secretly a villain
406. Original TV character; 10s; musician
409. From a TV show; based on comics; comics version a villain; 10s; DNA expert (both versions)
416. From a TV show; based on comics; 00s; scientist
418. From a movie; based on comics; 00s; physician
422. From a TV show; based on comics; 00s; police officer
423. From a TV show; based on comics; 00s; close friend of a superhero
425. Original TV character; 70s or 80s; athlete
427. Original TV character; 10s; jock/bully
431. From a TV show; based on comics; 00s; soldier
443. From a TV show; based on comics; 00s; name is a type of dish (in the food sense, not kitchenware sense)
444. From a movie; based on comics; 10s; ex-wife of superhero
448. From a TV show; based on comics; 00s; superspy
451. Original TV character; 90s; kid who befriended a superhero
452. Original TV character; 90s; fiancée of s villain
458. From a movie; based on comics; 10s; superhero's assistant
460. Original TV character; 10s; fan of comic books
471. Original TV character, subsequently used in movies; 90s; hunter of a specific type of monster
472. From a TV show; based on comics; 90s; girlfriend of superhero
477. Original movie character; 00s; girl who was in a coma
489. From a TV show; based on comics; 10s; mother of a superhero
493. Original TV character; 10s; bot-fighter
494. Original TV character; 10s; produced holograms
502. Original TV character; 10s; Asgardian (more or less)
507. From a movie; based on comics; 10s; mother of a character found in part 1 of Marvel Animated Other
510. From a TV show; based on comics; 90s
513. From a TV show; based on comics; 90s
515. From a TV show; based on comics; 00s
516. From a TV show; based on comics; 00s
517. From a TV show; based on comics; 00s
519. From a TV show; based on comics; 10s
520. From a TV show; based on comics; 90s
522. From a TV show; based on earlier show; 10s
528. From a TV show; based on comics; 00s
530. From a TV show; based on comics; 00s
532. Original TV character; 90s
533. From a TV show; based on comics; 10s
539. Original TV character; 00s
540. From a TV show; based on comics; 10s
541. Original TV character; 00s
551. From a TV show; based on comics; 10s
553. Original TV character but named after a real person. The real person with his name is a comic book writer; 90s
554. From a movie; based on comics; 00s
555. From a TV show; based on Asgardian mythology; 10s
560. From a TV show; based on comics; 90s
561. From a TV show; based on comics; 00s
562. Original TV characters; 10s
572. Original TV character; 60s
574. From a TV show; based on comics; 00s
579. From a TV show; based on comics; 10s
580. From a TV show; based on comics; 90s
581. From a TV show; based on comics; 70s or 80s
584. From a TV show; based on comics; 90s
588. Original TV character; 90s
600. From a TV show; based on comics; 10s
602. From a TV show; based on comics; 90s
610. Original TV character; 10s
611. Original TV character; 90s
612. Original TV character; 10s
617. From a TV show; based on comics; 90s
622. Original TV character; 10s
624. From a TV show; based on comics; 10s
626. From a TV show; based on comics; 70s or 80s
633. Original TV character; 10s
637. From a TV show; based on comics; 10s
638. Original TV character; 10s

Main character/team tally for remaining characters' shows/movies:
Spider-Man 27
X-Men 15
Avengers 10
Big Hero 6 5
Hulk 5
Guardians of the Galaxy 4
Iron Man 4
Doctor Strange 2
Fantastic Four 2
Super Hero Squad 2
Avengers (future) 1
Blade 1
Secret Warriors 1
Silver Surfer 1
Spider-Woman 1

I spy...
A character seated in an arena
A character in the education field who is not an educator in the comics
A character who scolded Spider-Man both in the show and in comics (not Jameson)
Two x-parents
At least five characters from other Earths in their shows or movies.
The father of a villain
 

477. Camille Carranza, Dr. Strange

355. Eldrac, Radomski

444. Mary Jane Watson, Spider-Verse—TRN701

Those are correct; list updated

Close enough to a day for more character specific clues. I'll probably add more before revealing the sources, but hopefully this will whittle things down a bit. As noted yesterday, you can now ask up to five of the same question per day if that further helps (20 questions total, can be boosted to 40 via bonus shots). Questions as always can be questions asked on previous days.

317. From a movie; based on comics; comics version more or less a hero. Comics debut: Strange Tales; Strange Tales debut was well after #100; 00s; extra-terrestrial resembling fantasy creature
322. From a TV show; based on comics; comics debut: X-Men; 00s; nickname is a vegetable
343. Original TV character; animated creators presumed Ralph Bakshi, Lin Carter, Fred Halliday, Ira Turek; from Spider-Man 1967; part of her name is a type of currency
351. Original TV character; in universe possibly created by Nova (Richard Rider); 10s; messenger droid
352. From a TV show; based on comics; human (altered). Assuming show follows comics, he was altered by taking a formula of his own creation; 10s; part of name describes a type of motion
360. From a TV show; based on comics; comics debut: New Mutants; 00s; name refers to a type of avalanche (but not based on Avalanche)
370. Original TV character but named after a real person. The real person with his name is usually a comic book writer, sometimes comics book artist; 10s; real person wrote a version of the comic that show was based on, with some controversial additions
372. From a TV show; based on comics; 00s; comics version has worn armour
373. From a TV show; based on comics; 10s; Asgardian, and name similar but not identical to someone from Norse mythology
377. From a TV show; based on comics; 10s; mother of a superhero
390. From a movie; based on comics; 00s; girlfriend of a superhero
396. From a TV show; based on comics; human (mutant); 00s; future version of a character who in the comics is a kid
399. From a movie; based on comics; 10s; sister of a superhero
403. From a TV show; based on comics; 00s; assistant to a CEO who's secretly a villain
406. Original TV character; 10s; musician
409. From a TV show; based on comics; comics version a villain; 10s; DNA expert (both versions)
416. From a TV show; based on comics; 00s; scientist
422. From a TV show; based on comics; 00s; police officer
423. From a TV show; based on comics; 00s; close friend of a superhero
425. Original TV character; 70s or 80s; athlete
427. Original TV character; 10s; jock/bully
431. From a TV show; based on comics; 00s; soldier
443. From a TV show; based on comics; 00s; name is a type of dish (in the food sense, not kitchenware sense)
448. From a TV show; based on comics; 00s; superspy
451. Original TV character; 90s; kid who befriended a superhero
452. Original TV character; 90s; fiancée of s villain
458. From a movie; based on comics; 10s; superhero's assistant
460. Original TV character; 10s; fan of comic books
471. Original TV character, subsequently used in movies; 90s; hunter of a specific type of monster
489. From a TV show; based on comics; 10s; mother of a superhero
493. Original TV character; 10s; bot-fighter
494. Original TV character; 10s; produced holograms
502. Original TV character; 10s; Asgardian (more or less)
507. From a movie; based on comics; 10s; mother of a character found in part 1 of Marvel Animated Other
510. From a TV show; based on comics; 90s; mother of a superhero
513. From a TV show; based on comics; 90s; parallel Earth version of a friend of a superhero
515. From a TV show; based on comics; 00s; imprisoned mutant
516. From a TV show; based on comics; 00s; ex-wife of a mutant and mother of another mutant
517. From a TV show; based on comics; 00s; wife and mother of two people with same alias
519. From a TV show; based on comics; 10s; brother of a vigilante
520. From a TV show; based on comics; 90s; parallel Earth version of a superhero's uncle
522. From a TV show; based on earlier show; 10s; scientist working for a task force
528. From a TV show; based on comics; 00s; mutant with some sort of machinery related ability
530. From a TV show; based on comics; 00s; step-daughter of a villain
532. Original TV character though loosely based on a male character; 90s; child with incurable illness
533. From a TV show; based on comics; 10s; father of a villain
539. Original TV character; 00s; sole survivor of hijackied plane
540. From a TV show; based on comics; 10s; resides with other monsters
541. Original TV character; 00s; teacher
551. From a TV show; based on comics; 10s; sister of superhero
553. Original TV character but named after a real person. The real person with his name is a comic book writer; 90s; energy drained by villain
554. From a movie; based on comics; 00s; nickname is a vegetable (is a counterpart of 322, though not from the same source)
555. From a TV show; based on Asgardian mythology; 10s; name and abilities resemble a Greek god
560. From a TV show; based on comics; 90s; father of a mutant
561. From a TV show; based on comics; 00s; name suggests anger management issues
562. Original TV characters; 10s; young bear-like creatures
572. Original TV character; 60s; no actual name; only known as another character's parent
574. From a TV show; based on comics; 00s; name aludes to breaking things
579. From a TV show; based on comics; 10s; mother of a mutant
580. From a TV show; based on comics; 90s; captured mutant
581. From a TV show; based on comics; 70s or 80s; police officer who encountered a superhero early on
584. From a TV show; based on comics; 90s; astronaut
588. Original TV character; 90s; supervisor at nuclear research facility
600. From a TV show; based on comics; 10s; name is a synonym for skirmish
602. From a TV show; based on comics; 90s; mutant mimic
610. Original TV character; 10s; professor/theorist
611. Original TV character; 90s; bone related code name
612. Original TV character; 10s; mother of a hero
617. From a TV show; based on comics; 90s; therapist
622. Original TV character; 10s; vampire hunter
624. From a TV show; based on comics (comics version a villain); 10s; this version a student
626. From a TV show; based on comics; 70s or 80s; scientist
633. Original TV character; 10s; extra-terrestrial scientist
637. From a TV show; based on comics; 10s; works for an Earth defense force
638. Original TV character; 10s; integrated into a hero team's headquarters

Main character/team tally for remaining characters' shows/movies:
Spider-Man 26
X-Men 15
Avengers 9
Big Hero 6 5
Hulk 5
Guardians of the Galaxy 4
Iron Man 4
Super Hero Squad 2
Avengers (future) 1
Blade 1
Fantastic Four 1
Secret Warriors 1
Spider-Woman 1

I spy...
A character seated in an arena
A character in the education field who is not an educator in the comics
A character who scolded Spider-Man both in the show and in comics (not Jameson)
Two x-parents
At least four characters from other Earths in their shows or movies

 

554. Pepper Potts, Invincible IM