For locked characters *=newly added.

LOCKED CHARACTERS:
25
55
92
128
170*
274
278*
288
341
351*
362
Some one-letter characters

PARTIALLY LOCKED CHARACTERS:
52
71*
108
136
198*
240
249
323
Some characters with one-letter code names

Other clues:
Two different characters played by the same actress decades appear consecutively.
There are six instances of a fictional/fictionalized character playing a fictional character, of which four are covered in the next point.
There are five characters from the Bond Girls skit mentioned in the first post, of which four are covered by rule 13.
There is one instance of the same character played by two actors in the same movie where both versions are in part 1. One has been identified, one has not.
Two consecutive characters phonetically (and with only one letter difference) give you the name of a major character from Agents of SHIELD


Tally (as before hiding some of the more obscure ones unless previously identified; with so many characters errors could arise)

Casino Royale 2006    29
Diamonds Are Forever    19
For Your Eyes Only    17
Octopussy    16
Licence to Kill    15
Tomorrow Never Dies    15
Casino Royale 1967    14
From Russia with Love    14
Quantum of Solace    14
On Her Majesty's Secret Service    13
Man with The Golden Gun    12
Thunderball    12
Goldfinger    11
Live and Let Die    11
Spy Who Loved Me    11
SNL    10
World Is Not Enough    10
Die Another Day    9
Dr. No    9
Spectre    9
The Living Daylights    9
GoldenEye    8
Skyfall    8
View to a Kill     8
No Time to Die    7
[Ian Fleming biopic]    6
You Only Live Twice    6
Never Say Never Again    5
Climax Theater: Casino Royale    3
Moonraker    3
[Ian Fleming miniseries]    2
[Ian Fleming biopic]    2
[Ian Fleming biopic]     2
[sketch show]    2
[sketch show]    1
[fundraiser show]     1
[martial arts movie]    1
James Bond and the Queen    1
[talk show]    1
[movie about master thief]    1
[soft drink commercial]    1
[talk show]    1
[short film within sketch show of sorts]    1