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253. Ben Parker, TAS—98311
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257. Koh, Anime
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145. Miyuki, Anime
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151. Ho Yinsen, Armored Adventures
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97. Isabela Vanko, Armored Adventures
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146 is incorrect. The rest are correct. List updated. 112: I accidentally deleted the wrong number. Restored that one and removed the proper character..
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115. Betty Ross (who looks like she's taken a little gamma radiation herself), The MSH
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155. Celia Rawlings, The MSH
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275. Chester Phillips, The MSH
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313. Neptune, The MSH
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168. Peggy Carter, The MSH
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72. Seidring, The MSH
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164. Sharon Carter, The MSH
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182. Anelle, FF 67
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312. Pearla, FF67
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192. Eric Schwinner, SM 67
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Those are correct. List updated. While Marvel Animated Wiki is a good place to find images of otherwise hard to find early animated characters, you can tell at times from the colours that they weren't grabbing the images from the Region 2 MSH DVDs.
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Just to recap a few things, zuckyd1 started with 51 bonus shots and used 40 but gained 25, leaving 36. Okay to start asking questions. Yes/No questions with count towards your daily 20 questions but not towards total shots for the day. Other questions will count towards both as usual.
First set of source clues:
1. From a TV show; based on comics
2. From a TV show; based on comics
3. Original TV character
6. Original TV character I think
7. From a TV show; based on another show
8. Original TV character I think but related to character based on comics
9. From a TV show; based on comics
13. From a TV show; based on comics
15. Original TV character
17. From a TV show; based on comics
18. From a TV show; based on comics
19. From a TV show; based on comics
20. Original TV character
22. Original TV character
29. From a TV show; based on movie
33. Original movie character
34. From a movie; based on comics
35. From a TV show; based on comics
40. From a TV show; based on comics (different first name in comics)
43. From a TV show; based on comics
 
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187. Abigail Callaghan, BH6
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34. Ganke Lee, Spider-Verse
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126. Cletus Kasady, Spectacular SM
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127. Ho Yin, Invincible IM
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288. Elijah, Dracula
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52. Frank Drake, Dracula
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267. Rachel Van Helsing, Dracula
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263. Dolores, Dracula?
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227. Gus, BH6
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309. Bolton Gramercy, BH6
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Those are correct. List updated.
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Who created the animated versions of the first 20 characters?