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110. Dick Clark, Action Hour
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111. Rora, Radomski
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133. Dar Mal Goth, Ultimate
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124. Tully, Radomski
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139. Eitri, Yost
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141. Euclid, Ultimate
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147. Paul?, Evolution
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190. Lucy, Radomski
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212. Girkland, TAS
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235. Agent Machida, Anime
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269. George Stacy, Rising
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270. Aneka, Ultimate
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296. Mr. Ohare, BH6
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299. Rebel Ralston?, TAS
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303. Recording Observation Bot, Ultimate
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308. J. Joanna Jameson, Ultimate—TRN454
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108 is incorrect; someone a lot more obscure even than Corbeau (and of course Corbeau isn't an original TV character). Paul I'll allow (his surname is Haits). The rest are correct. List updated.
BTW while I wasn't able to find a Marvel specific comics appearance, I found some non-Marvel appearances. It appears that his comics debut was in DC's Pat Boone #5, appropriately given his love of music.
Jameson is worth 5 bonus shots, so zuckyd1 now has 65.
57. Original TV character; John Semper
67. Original TV character; presumabbly Cosmo Anzilotti, Ralph Bakshi, Gray Morrow, Grant Simmons, Ralph Bakshi, Lin Carter, Fred Halliday, Ira Turek; episode described as down to earth
108. Original TV character; presumed Stéphane Juffé, Christopher Yost
205. presumed Larry Parr; Fantastic Four by Stan Lee, Jack Kirby
209. Mark Edward Edens; X-Men by Chris Claremont, Dave Cockrum
218. presumed Donald F. Glut; X-Men by Gary Friedrich, George Tuska, John Verpoorten
223. presumed Mairghread Scott; n/a
234. Jan Strnad; (different name) Avengers by Roy Thomas, Neal Adams
258. presumed Don Jurwich, Bob Richardson, Donald F. Glut; it's complicated but let's go with Defenders by Steve Englehart, Sal Buscema
260. Cydne Clark, Steve Granat; (code name only) Marvel Team-Up by Chris Claremont, John Byrne
268. Yugo Serikawa; Toyoo Ashida, Akiyoshi Sakai; n/a
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108. John, Armored Adventures
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189 should be removed
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209. Moira MacTaggert, TAS
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234. Mandroids, Action Hour
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260. Arcade, Evolution
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268. Bellbeau?, Frankenstein
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268 isn't Bellebeau but you're very, very close. The rest are correct. List updated. Only seven left!
As a reminder of the current 20 Questions rules, you can still ask up to 20 questions a day, and up to three a day can be the same question. Also okay to "rotate" questions (ask a question for three characters one day, and then the next day ask another three those questions).
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Almost a day so since there's only seven left, adding the source, main character, and decades clues. Since the source clues make the decades obvious between 70s and 80s, splitting those this time around.
57. Original TV character; John Semper; 90s
67. Original TV character; presumabbly Cosmo Anzilotti, Ralph Bakshi, Gray Morrow, Grant Simmons, Ralph Bakshi, Lin Carter, Fred Halliday, Ira Turek; episode described as down to earth; 60s
205. From a TV show; based on comics; presumed Larry Parr; Fantastic Four by Stan Lee, Jack Kirby; 80s
218. From a TV show; based on comics; presumed Donald F. Glut; X-Men by Gary Friedrich, George Tuska, John Verpoorten; 80s
223. Original TV character; presumed Mairghread Scott; 10s
258. From a TV show; based on comics; presumed Don Jurwich, Bob Richardson, Donald F. Glut; it's complicated but let's go with Defenders by Steve Englehart, Sal Buscema; 80s
268. Original movie character; Yugo Serikawa; Toyoo Ashida, Akiyoshi Sakai; n/a; not Belleveau's assistant but very, very close; 70s
Main characters
Spider-Man 5
Frankenstein's Monster 1
Secret Warriors 1
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268 Bellbeau's assistant, Monster of Frankenstein.
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258 Valkyrie, Spider-Man and his Amazing Friends
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57 Karl (possibly Groitzig, since that's his wife's surname), Spider-Man Animated (90s)
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67 Osa Olsen, Spider-Man 1967
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Those are correct. List updated. I was being playful when I described Osa's episode: Down to Earth is the title of the episode. Three left, one of whom has the 20 bonus shots. Two of them have something interesting in common beyond the source.