24 Swami Armada, The Adventures of Superman (1950s)

123 Joker, Vai que... Batman

200 Wonder Woman, Powergen advert?

295 Madame Rouge, Doom Patrol

200 isn't from that source; since that one's one of the tougher ones I'll confirm that it is Wonder Woman. The rest are correct; list updated.

273 Professor Arnold Leeds, Superman (1948) serial

193 isn't Nolan nor that serial; Klein I'll allow (I think you meant 57) 273 is correct; list updated.

Adding character specific clues to 112.

1. From a TV show; based on comics; 10s; Detective Comics; from non-SNL skit    
5. Original TV show character; 20s; some sort of scientist
7. Original movie character; 10s; interview subject
8. From a TV show; based on comics, though surname from earlier show; 20s; Batman (surname: Gotham); Appears to lack the ties to a major supporting character that other versions of this character have
11. From a TV show; based on comics; 20s; Flash Comics; villain turned anti-hero
13. Original movie character; 10s; crime syndicate leader
14. Original TV show character (a comics character with the same name appeared months earlier but this appears to have been a coincidence); 80s; son of literary character
15. Original movie character; 00s; attempted to assault voters at polling station
16. From a TV show; based on comics; 90s; Superman's Girlfrioend Lois Lane; sister of major villain
17. From a movie; based on comics; 10s; Superman; scientist
18. From a TV show; based on comics (while comics characters with the same code name appeared earlier, this is more a gender swapped swapped version of another character); 60s; Superman (earliest use of code name), Action Comics (character most associated with code name, plus debut of the gender swapped character); from a comedy skit
19. Original movie character; 20s; dictator
20. Original TV show character; 10s; able to fit into tight spaces
22. From a movie; based on comics; 00s; Watchmen; owned some sort of small business
26. Original movie character; 00s; judge with mob ties
27. Original TV show character; 10s; circus performer
28. From a TV show; based on comics; 00s; Action Comics; extra-terrestrial villain    
30. From a movie; has same name as comics character but this may be a coincidence; 10s; possibly Adventure Comics; unemployed mother
31. Original movie character; 90s; brother of someone who soon became a superhero
32. Original TV show character; 00s; biological dad of major character
34. From a TV show; based on comics; 10s; Catwoman; mother of major character
37. Original TV show character; 10s; cheerleader
38. From a movie; loosely based on comics; 80s; Action Comics; principal
39. Original TV show character; 80s; has flight and energy projection
40. Original TV show character; 70s; newspaper man
41. From a TV show; based on comics; 10s; New Teen Titans; ex-wife of major villain
42. Original TV show character; 10s; high school football player
43. Original TV show character; 10s; daughter of major character
44. From a TV show; based on comics; 10s; My Greatest Adventure; superhero
45. Original TV show character; 10s; ex-model
46. From a commercial; based on comics; 10s; Detective Comics; seen in three commercials
48. Original movie character; 10s; janitor
49. Original movie character; 90s; mayor
53. From a movie; based on comics; 00s; Batman; wife of major supporting character
55. From a TV show; based on earlier character same continuity; 80s; scientist
56. Original TV show character; 00s; FBI agent
57. Original TV show character; 90s; scientist
58. Original TV show character; 70s; environmentalist chemist with powers
59. From a TV show; based on comics; 20s; Justice League America; possessed by counterpart
60. Original TV show character; 60s; ran health spa
61. Original TV show character; 80s; professional thief
64. From a TV show; based on comics; 10s; Detective Comics; acrobat
67. Original movie character; 10s; newspaper reporter
68. From a movie; based on comics; 70s; Superman's Girlfriend Lois Lane; seen on train
69. Original TV show character; 20s; has some sort of connection to time
70. From a commercial; claims to be character based on comics; 10s; Detective Comics if you take his claims seriously; seen in Japanese commercial
71. From a TV show; based on earlier show; 10s; The Batman cartoon; worked in toy store
72. Original TV show character; 00s; wired himself with a bomb
73. Original movie character; 10s; a bully
74. From a TV show; based on comics; 10s; Detective Comics; adoptive father of main character
75. From a movie; loosely based on character from TV show; 20s; Batman (1960s series); police commissioner
76. From a TV special, based on stage version; 70s; mafia leader
77. From a movie; based on comics; 00s; Detective Comics; majopr antagonist
79. From a commercial; based on comics; 00s?; supporting character
80. Original movie character; 10s; planetary leader
82. Original TV show character; 60s; favoured automatic guns
84. From a TV show; based on earlier character same continuity; 20s; extra-terrestrial
86. Original TV show character; 00s?; rain engineering company
88. From a TV show; based on comics; 10s; DC Comics Presents; magical character
89. Original TV show character; 00s; medical doctor
91. Original TV show character; 10s; police sergeant
93. Original TV show character; 70s; wanted to get her brother out of the drug world
94. Original TV show character; 80s; sought revenge for father's execution
95. Original TV show character; 80s; homeless former classmate of main character
96. Original TV show character; 10s; nurse
97. Original TV show character; 00s; part of British royalty
98. Original TV show character; 10s; part of resistance group
99. Original TV show character; 10s; lead scientist for major villain
101. Original movie character; 00s; special ops captain
102. Original TV show character; 10s; news reporter
103. Original TV show character; 90s; newscaster I think (hard to find info online and it's been decades since I've watched anything with the character)
104. Original TV show character; 70s; accidentally helps start a fire
106. Original TV show character; 10s; mother of main character
111. From a movie; code name based on comics; 90s; Detective Comics code name only; dressed as superhero to go after people dressed as villains
112. From a movie; based on comics; 00s; Batman: Shadow of the Bat; kills for the mob
113. From a movie; based on comics; 20s; Gotham Central
114. Original TV show character; 70s
115. From a TV show; based on comics; 10s; Hellblaer
117. Original TV show character wearing comics based costume; 50s
118. Original TV show character; 80s
119. From a TV show; based on comics; 10s; Black Lightning
120. Original movie character; 80s
121. Original TV show character; 80s
122. Original TV show character; 20s
124. From a TV show; based on comics; 90s; Action Comics
125. From a TV show; based on earlier character same continuity; 90s
127. From a movie; dressed as character based on comics; 00s (with caveat); dresseed as character from Action Comics
130. Original TV show character; 10s
132. Original TV show character; 00s
133. From a TV show; based on comics; 10s; Infinity Inc.
136. From a TV show; somewhat based on comics (different first name); 20s; Secret Origins
138. Original TV show character; 80s
139. Original TV show character; 70s
140. Original TV show character; 20s
142. Original TV show character; 60s
143. Original TV show character but claimed to be comics based character; 20s
144. From a TV show; based on comics; 80s; Doom Patrol
146. Original movie character; 20s
147. From a TV show; based on comics; 20s; Justice League America
149. From a movie; somewhat based on comics; 70s; loosely Action Comics
150. From a TV show; based on comics; 10s; Swamp Thing
152. Original TV show character; 10s
153. From a TV special, based on stage version; 70s
154. From a movie; based on comics; 10s; Gotham Central
156. From a TV show; based on comics; 10s; Detective Comics
158. From a TV show; based on comics; 70s; Action Comics
159. Original TV show character; 80s
160. Original movie character; 10s
163. Original TV show character; 10s
166. From a TV show; based on comics; 90s; Green Lantern
168. From a TV show; based on comics; 10s; Infinity Inc.
169. From a commercial; based on comics; 10s; Action Comics
172. Original TV show character; 10s
173. Original TV show character;m 10s
174. From a TV show; based on comics; 00s; Infinite Crisis
177. From a TV show; based on comics; 10s; Hellblazer
178. Original TV show character; 10s
179. From a commercial; based on comics; 90s; Action Comics
180. Original movie character; 10s
183. Original TV show character; 80s
184. From a movie; based on comics; 10s; The Atom
187. Original TV show character; 70s
188. Original TV show character; 10s
190. From a TV show; based on comics, though named on radio show; 90s; Action Comics, named on The Adventures of Superman
191. From a TV show; based on comics; 10s; Justice League of America
192. Original movie character; 10s
193. Original movie serial character; 40s
196. From a TV show; based on comics; 10s; Black Lightning
199. From a movie; based on comics; 90s; Detective Comics
200. From a commercial; based on comics; 90s [correctly identified as Wonder Woman but need to ID where from]
201. Original TV show character; 00s
202. From a movie; based on TV show; 10s; Smallville
203. From a movie; based on comics; 10s; Superman
204. From a movie; based on comics; 70s; Superman's Girlfriend Lois Lane
205. Original TV show character; 10s
207. From a TV show; based on comics (first name from earlier show); 10s; Superman's Girlfriend Lois Lane (first name from Smallville)
208. Original TV show character; 60s
209. Original movie character; 10s
210. Original TV show character; 60s
211. Original movie character but likely named after real person; 10s
212. From a commercial; based on comics; 70s; All Star Comics
213. Original movie character; 60s
214. Original movie character; 80s
215. Original TV pilot character, though comics characters have similar code names; 90s
218. Original movie character; 10s
219. Original movie character; 00s
220. Original TV show character; 10s
226. From a TV show; based on comics; 10s; Infinity Inc
228. Original TV show character; 70s
229. Original movie serial character; 50s
230. Original TV show character; 60s
234. Original TV show character; 10s
236. From a TV show; based on comics; 10s; Batman
237. Original TV show character; 10s
239. Original TV show character; 10s
240. Original TV show character; 80s
241. From a TV show; based on comics; 10s; Hawk and Dove
243. From a TV show; based on comics; 20s; Batman: The Dark Knight Returns
244. Original TV show character, though comics have a character with the same name; 10s
245. From a movie; based on comics; 00s; Batman
249. From a TV show; based on comics; 00s; Batman and the Outsiders
251. From a TV show; based on comics; 00s; Action Comics
255. From a TV show; based on comics; 10s; The Phantom Stranger
256. From a TV show; based on comics; 80s; Superboy
259. From a TV show; based on comics; 80s; Superman
260. From a TV show; based on comics; 10s; DC Comics Presents
261. From a TV show; based on comics; 20s pictured but first mentioned in 10s; Detective Comics
262. From a movie; based on comics; 90s; Detective Comics
264. From a TV show; loosely based on comics; 10s; Detective Comics
265. From a TV show; based on comics; 10s; DC Comics Presents
266. From a TV show; based on comics; 20s; The Flash
267. Original movie character; 90s
268. Original movie character; 80s
269. From a TV show; based on comics; 90s; Detective Comics
270. From a TV show; based on comics; 00s; Forever People
271. From a movie; based on comics; 10s; Action Comics
274. Original TV show character; 10s
277. From a TV show; based on comics; 10s; Detective Comics
278. From a TV pilot; based on comics; 10s; All Star Comics
279. From a TV show; based on comics; 10s; Infinity Inc.
280. From a TV show; based on comics; 10s; Batman: Shadow of the Bat
281. From a movie; based on comics; 10s; Justice League
282. Original TV show character; 00s
286. From a TV show; based on comics; 10s; Teen Titans
288. Original TV show character; 10s
289. Original TV show character; 10s
290. From a TV show; based on comics; 90s; Action Comics
291. Original TV show character; 10s
292. From a movie; based on comics; 10s; Wonder Woman
293. Original TV show character; 70s
296. From a TV show; based on comics, though comics version debuted with different first name; 80s; Superboy
297. Original TV show character; 90s
301. Original movie character; 00s
302. From a TV show; based on comics; 10s; Infinity Inc.
303. Original movie character; 10s
304. From a movie; based on comics; 70s; Action Comics
307. From a movie; superhero name/look based on comics; 80s; Action Comics
308. From a movie; based on comics; 10s; The Man of Steel
311. From a movie; loosely based on comics; 70s; Detective Comics
312. Original movie character; 20s
316. Original TV show character, though comics have character of same name; 80s
321. Original movie character; 10s
325. Original TV show character; 60s
326. Original TV show character; 10s
327. Original movie character; 00s
329. From a TV special; based on stage version; 70s
330. Original TV show character; 80s
331. Original TV show character; 10s
332. Original TV show character; 90s

Adding remaining character specific clues.

1. From a TV show; based on comics; 10s; Detective Comics; from non-SNL skit    
5. Original TV show character; 20s; some sort of scientist
7. Original movie character; 10s; interview subject
8. From a TV show; based on comics, though surname from earlier show; 20s; Batman (surname: Gotham); Appears to lack the ties to a major supporting character that other versions of this character have
11. From a TV show; based on comics; 20s; Flash Comics; villain turned anti-hero
13. Original movie character; 10s; crime syndicate leader
14. Original TV show character (a comics character with the same name appeared months earlier but this appears to have been a coincidence); 80s; son of literary character
15. Original movie character; 00s; attempted to assault voters at polling station
16. From a TV show; based on comics; 90s; Superman's Girlfrioend Lois Lane; sister of major villain
17. From a movie; based on comics; 10s; Superman; scientist
18. From a TV show; based on comics (while comics characters with the same code name appeared earlier, this is more a gender swapped swapped version of another character); 60s; Superman (earliest use of code name), Action Comics (character most associated with code name, plus debut of the gender swapped character); from a comedy skit
19. Original movie character; 20s; dictator
20. Original TV show character; 10s; able to fit into tight spaces
22. From a movie; based on comics; 00s; Watchmen; owned some sort of small business
26. Original movie character; 00s; judge with mob ties
27. Original TV show character; 10s; circus performer
28. From a TV show; based on comics; 00s; Action Comics; extra-terrestrial villain    
30. From a movie; has same name as comics character but this may be a coincidence; 10s; possibly Adventure Comics; unemployed mother
31. Original movie character; 90s; brother of someone who soon became a superhero
32. Original TV show character; 00s; biological dad of major character
34. From a TV show; based on comics; 10s; Catwoman; mother of major character
37. Original TV show character; 10s; cheerleader
38. From a movie; loosely based on comics; 80s; Action Comics; principal
39. Original TV show character; 80s; has flight and energy projection
40. Original TV show character; 70s; newspaper man
41. From a TV show; based on comics; 10s; New Teen Titans; ex-wife of major villain
42. Original TV show character; 10s; high school football player
43. Original TV show character; 10s; daughter of major character
44. From a TV show; based on comics; 10s; My Greatest Adventure; superhero
45. Original TV show character; 10s; ex-model
46. From a commercial; based on comics; 10s; Detective Comics; seen in three commercials
48. Original movie character; 10s; janitor
49. Original movie character; 90s; mayor
53. From a movie; based on comics; 00s; Batman; wife of major supporting character
55. From a TV show; based on earlier character same continuity; 80s; scientist
56. Original TV show character; 00s; FBI agent
57. Original TV show character; 90s; scientist
58. Original TV show character; 70s; environmentalist chemist with powers
59. From a TV show; based on comics; 20s; Justice League America; possessed by counterpart
60. Original TV show character; 60s; ran health spa
61. Original TV show character; 80s; professional thief
64. From a TV show; based on comics; 10s; Detective Comics; acrobat
67. Original movie character; 10s; newspaper reporter
68. From a movie; based on comics; 70s; Superman's Girlfriend Lois Lane; seen on train
69. Original TV show character; 20s; has some sort of connection to time
70. From a commercial; claims to be character based on comics; 10s; Detective Comics if you take his claims seriously; seen in Japanese commercial
71. From a TV show; based on earlier show; 10s; The Batman cartoon; worked in toy store
72. Original TV show character; 00s; wired himself with a bomb
73. Original movie character; 10s; a bully
74. From a TV show; based on comics; 10s; Detective Comics; adoptive father of main character
75. From a movie; loosely based on character from TV show; 20s; Batman (1960s series); police commissioner
76. From a TV special, based on stage version; 70s; mafia leader
77. From a movie; based on comics; 00s; Detective Comics; majopr antagonist
79. From a commercial; based on comics; 00s?; supporting character
80. Original movie character; 10s; planetary leader
82. Original TV show character; 60s; favoured automatic guns
84. From a TV show; based on earlier character same continuity; 20s; extra-terrestrial
86. Original TV show character; 00s?; rain engineering company
88. From a TV show; based on comics; 10s; DC Comics Presents; magical character
89. Original TV show character; 00s; medical doctor
91. Original TV show character; 10s; police sergeant
93. Original TV show character; 70s; wanted to get her brother out of the drug world
94. Original TV show character; 80s; sought revenge for father's execution
95. Original TV show character; 80s; homeless former classmate of main character
96. Original TV show character; 10s; nurse
97. Original TV show character; 00s; part of British royalty
98. Original TV show character; 10s; part of resistance group
99. Original TV show character; 10s; lead scientist for major villain
101. Original movie character; 00s; special ops captain
102. Original TV show character; 10s; news reporter
103. Original TV show character; 90s; newscaster I think (hard to find info online and it's been decades since I've watched anything with the character)
104. Original TV show character; 70s; accidentally helps start a fire
106. Original TV show character; 10s; mother of main character
111. From a movie; code name based on comics; 90s; Detective Comics code name only; dressed as superhero to go after people dressed as villains
112. From a movie; based on comics; 00s; Batman: Shadow of the Bat; kills for the mob
113. From a movie; based on comics; 20s; Gotham Central; corrupt detective
114. Original TV show character; 70s; from other-dimensional land
115. From a TV show; based on comics; 10s; Hellblaer; former friend of main character
117. Original TV show character wearing comics based costume; 50s; met either a superhero or the actor playing the superhero
118. Original TV show character; 80s; helped maintain boat house
119. From a TV show; based on comics; 10s; Black Lightning; counterpart of major supporting character
120. Original movie character; 80s; shares surname with popular real life 1980s comedian
121. Original TV show character; 80s; corrupt DA
122. Original TV show character; 20s; high school Class President
124. From a TV show; based on comics; 90s; Action Comics; seen in non-English language comedy skit
125. From a TV show; based on earlier character same continuity; 90s; counterpart of recurring villain and herself a villain
127. From a movie; dressed as character based on comics; 00s (with caveat); dresseed as character from Action Comics; real life documentary subject
130. Original TV show character; 10s; possessed by murderer
132. Original TV show character; 00s; FBI agent
133. From a TV show; based on comics; 10s; Infinity Inc.; son of super-villain
136. From a TV show; somewhat based on comics (different first name); 20s; Secret Origins; mother of woman with powers
138. Original TV show character; 80s; weapons dealer
139. Original TV show character; 70s; German intelligence officer
140. Original TV show character; 20s; potential love interest of supporting character
142. Original TV show character; 60s; criminal stage magician
143. Original TV show character but claimed to be comics based character; 20s; impersonated a major character for an extended period
144. From a TV show; based on comics; 80s; Action Comics; good counterpart of a villain
146. Original movie character; 20s; party attendee
147. From a TV show; based on comics; 20s; Justice League America; government agent who didn't get along with main character
149. From a movie; somewhat based on comics; 70s; loosely Action Comics; Christmas ornaments play a role in his learning the truth about himself
150. From a TV show; based on comics; 10s; Swamp Thing; doctor specializing in diseases
152. Original TV show character; 10s; blessed citizens
153. From a TV special, based on stage version; 70s; repeatedly lost Nobel Prize
154. From a movie; based on comics; 10s; Gotham Central; police officer
156. From a TV show; based on comics; 10s; Detective Comics; caused deaths of major character's parents
158. From a TV show; based on comics; 70s; Action Comics; from skit
159. Original TV show character; 80s; targeted so father wouldn't testify against someone
160. Original movie character; 10s; former landlord of major character
163. Original TV show character; 10s; mother of villain/anti-hero
166. From a TV show; based on comics; 90s; Green Lantern; from skit
168. From a TV show; based on comics; 10s; Infinity Inc.; father of superhero
169. From a commercial; based on comics; 10s; Action Comics; seen in department store commercial
172. Original TV show character; 10s; senator/son of comics based character
173. Original TV show character; 10s; fortune teller
174. From a TV show; based on comics; 00s; Infinite Crisis; superhero (legacy hero in comics but not in show)
177. From a TV show; based on comics; 10s; Hellblazer; drug addict
178. Original TV show character; 10s; explored other realities
179. From a commercial; based on comics; 90s; Action Comics; commercial has some sort of Texas focus
180. Original movie character; 10s; played a hand in a massacre
183. Original TV show character; 80s; museum PR
184. From a movie; based on comics; 10s; Batman; son might become a superhero someday
187. Original TV show character; 70s; competed for main character's title
188. Original TV show character; 10s; uncle of major supporting character
190. From a TV show; based on comics, though named on radio show; 90s; Action Comics, named on The Adventures of Superman; from skit
191. From a TV show; based on comics; 10s; Justice League of America; magic based villain
192. Original movie character; 10s; brother of main villain
193. Original movie serial character; 40s; lab henchman
196. From a TV show; based on comics; 10s; Black Lightning; right hand man of main villain
199. From a movie; based on comics; 90s; Detective Comics; girlfriend of main character
200. From a commercial; based on comics; 90s [correctly identified as Wonder Woman but need to ID where from]; commercial has some sort of Texas focus
201. Original TV show character; 00s; counter-intelligence officer
202. From a movie; based on TV show; 10s; Smallville; bully
203. From a movie; based on comics; 10s; Superman; mother of main character
204. From a movie; based on comics; 70s; Superman's Girlfriend Lois Lane; seen on train
205. Original TV show character; 10s;' police detective
207. From a TV show; based on comics (first name from earlier show); 10s; Superman's Girlfriend Lois Lane (first name from Smallville); former of major villain
208. Original TV show character; 60s; helped teach young girls to become thieves
209. Original movie character; 10s; police captain
210. Original TV show character; 60s; would-be alchemist
211. Original movie character but named after real person; 10s; newscaster
212. From a commercial; based on comics; 70s; All Star Comics; from department stores commercial
213. Original movie character; 60s; pirate-themed henchman
214. Original movie character; 80s; news anchor
215. Original TV pilot character, though comics characters have similar code names; 90s; main villain in the pilot
218. Original movie character; 10s; nightclub owner
219. Original movie character; 00s; scientist
220. Original TV show character; 10s; captive meta-human
226. From a TV show; based on comics; 10s; Infinity Inc; mother of superhero
228. Original TV show character; 70s; daughter of supporting character
229. Original movie serial character; 50s; villains wanted his invention
230. Original TV show character; 60s; villainous chanteuse
234. Original TV show character; 10s; part of rebel organization
236. From a TV show; based on comics; 10s; Batman; smuggler
237. Original TV show character; 10s; maid
239. Original TV show character; 10s; orchestrated a massacre
240. Original TV show character; 80s; friend of main character
241. From a TV show; based on comics; 10s; Hawk and Dove; pedophile
243. From a TV show; based on comics; 20s; Batman: The Dark Knight Returns; potential future recruit for one hero
244. Original TV show character, though comics have a character with the same name; 10s; markswoman, has fast reflexes
245. From a movie; based on comics; 00s; Batman; key figure in main character
s origin
249. From a TV show; based on comics; 00s; Batman and the Outsiders; chemist who has trouble relating to people
251. From a TV show; based on comics; 00s; Action Comics; leader of biker gang
255. From a TV show; based on comics; 10s; The Phantom Stranger; enigmatic guide
256. From a TV show; based on comics; 80s; Superboy; sorceress
259. From a TV show; based on comics; 80s; Superman; mother of main character
260. From a TV show; based on comics; 10s; DC Comics Presents; seen in non-SNL skit
261. From a TV show; based on comics; 20s pictured but first mentioned in 10s; Detective Comics; worked with one of the main characters in the past
262. From a movie; based on comics; 90s; Detective Comics; mother of superhero
264. From a TV show; loosely based on comics; 10s; Detective Comics; main character in a show from an African country
265. From a TV show; based on comics; 10s; DC Comics Presents; this version only seen on magazine cover
266. From a TV show; based on comics; 20s; The Flash; can command a magical being
267. Original movie character; 90s; one of a group of similar criminals seen at the start of the movie
268. Original movie character; 80s; testified against drug lord
269. From a TV show; based on comics; 90s; Detective Comics; always works with brother
270. From a TV show; based on comics; 00s; Forever People; main lackey for villain
271. From a movie; based on comics; 10s; Action Comics; war criminal
274. Original TV show character; 10s; crime family member
277. From a TV show; based on comics; 10s; Detective Comics; seen in non-SNL skit
278. From a TV pilot; based on comics; 10s; All Star Comics; key part of main character's origin
279. From a TV show; based on comics; 10s; Infinity Inc.; duaghter of super-villains
280. From a TV show; based on comics; 10s; Batman: Shadow of the Bat; asylum inmate
281. From a movie; based on comics; 10s; Justice League; foster mother of main character
282. Original TV show character; 00s; mother of major character
286. From a TV show; based on comics; 10s; Teen Titans; mobster
288. Original TV show character; 10s; colonel
289. Original TV show character; 10s; pastor/healer
290. From a TV show; based on comics; 90s; Action Comics; from skit
291. Original TV show character; 10s; terrorist posing as model
292. From a movie; based on comics; 10s; Wonder Woman; warrior
293. Original TV show character; 70s; spy for an enemy country
296. From a TV show; based on comics, though comics version debuted with different first name; 80s; Superboy; father of major character
297. Original TV show character; 90s; reformed burglar
301. Original movie character; 00s; apparent son of main character
302. From a TV show; based on comics; 10s; Infinity Inc.; son of major character
303. Original movie character; 10s; colonel who aided main character
304. From a movie; based on comics; 70s; Action Comics; from non-US parody movie
307. From a movie; superhero name/look based on comics; 80s; Action Comics; seen in costume in musical number
308. From a movie; based on comics; 10s; The Man of Steel; service robot
311. From a movie; loosely based on comics; 70s; Detective Comics; main character of three movies (with co-star)
312. Original movie character; 20s; corrupt city councilman
316. Original TV show character, though comics have character of same name; 80s; name refers to type of small mammal
321. Original movie character; 10s; owned unusual pet store
325. Original TV show character; 60s; husband of 210
326. Original TV show character; 10s; named after major character
327. Original movie character; 00s; patrolman
329. From a TV special; based on stage version; 70s; name is same as Australian city
330. Original TV show character; 80s; blind newspaper seller
331. Original TV show character; 10s; nun/ex-lover of main character
332. Original TV show character; 90s; rival of one of the main characters

I've decided to tally the sources of remaining characters. In some cases (because they're from from continuities not previously seen this year or 2020) I've opted to put a clue instead of identifying them. In such cases the clue is in brackets.

Gotham 13
Black Lightning 11
Superboy 11
Constantine TV 10
Flash 1990 9
Stargirl 9
Titans 9
Birds of Prey 7
Man of Steel 7
Smallville 7
Wonder Woman tv 7
Batman (1960s) 6
Human Target (2010) 6
Krypton 5
Shazam! movie 5
Lois & Clark 4
SNL (various) 4
Superman and Lois 4
The Batman (2022) 4
Batman Begins 3
[skit related to comics related event] 3
It's a Bird, it's a Plane, It's Superman 3
The Dark Knight 3
Batman Forever 2
Batman Returns 2
Justice League movie (either cut) 2
Shazam! TV 2
[live show commerical] 2
Superman: The Movie 2
Swamp Thing (2019) 2
Swamp Thing movie 2
The Dark Knight Rises 2
Watchmen (TV) 2
[characters pose as heroes or villains] 1
Atom Man vs Superman 1
Batman & Robin (1997) 1
Batman (1989) 1
[game commercial] 1
Batman OnStar commercial 1
Batman v Superman: Dawn of Justice 1
Batman: The Movie 1
Batwoman 1
[movie with sidekick normally associated with different hero] 1
Blackhawk 1
Bradesco Seguros commercials 1
Catwoman 1
[foreign skit series] 1
[documentary] 1
Doom Patrol 1
Flash (modern) 1
[early skit show] 1
[popular sitcom] 1
[award winning movie] 1
Justice League of America pilot 1
Legends of Tomorrow 1
Naomi 1
Pennyworth 1
Powerless 1
Supergirl movie 1
Superman Returns 1
Swamp Thing (1990s series) 1
[African TV series] 1
[foreign movie with title similar to US movie] 1
The Suicide Squad (2021) 1
[movie has song and dance sequence with Marvel related character] 1
[commercial tied to event kids hate] 1
Watchmen movie 1
Wonder Woman (2011 pilot) 1
Wonder Woman movie 1
[commercial for two store chains] 1
[movie has sound effect in name] 1

18 Supergirl, Carol Burnett Show

22 Bernard, Watchmen movie

191 Felix Faust, Constantine

64 John Grayson, Gotham

106 Chary-El, Krypton