174 Sister Boy, Luke Cage

118 Riley, Winter Soldier

149 Davis, Days of Future Past

Latest answers correct; list updated

126. Spider-Man, Cingular Wireless commercial?

158. Pearson, Ant-Man & Wasp

Added next 60 character specific clues

12. Original movie character; 10s; member of criminal syndicate
13. Original TV show character; 10s; bank manager
15. Original movie character; 10s; hosted science videos
20. Original movie character; 00s; pulled gun on main character
28. Original TV show character; 10s; former member of terrorist group
29. Original TV show character; 10s; performed experiments on people
31. Original TV show character; 70s; reporter
33. Original TV show character; 10s; assisted in battle with artificial being
35. Original TV show character; 10s; student who attended lecture by major characters
40. Original TV show character; 10s; saved from rape attempt
43. Original movie character; 00s; medical doctor
44. Original TV show character; 10s; helped rescue major character while the latter was an infant
46. Original movie character; 00s; senator
50. Original movie character; 10s; high school teacher
51. Original TV show character; 10s; criminal figure
55. Original movie character; 10s; info sparse on this character and I don't recall them, but we only know her first name
56. From a commercial; based on comics; 00s; Captain America Comics; seen in underground base
59. Original TV show character; 10s; drug dealer
77. Original TV show character; 10s; embezzler
78. Original TV show character; 10s; has power of biological manipulation
79. Original TV show character; 20s; drove multiple major characters
84. Original TV show character; 10s; pilot
91. Original TV show character; 10s; police detective
101. Original TV show character; 10s; astronomer
108. Original TV show character; 10s; police captain
109. Original TV show character; 10s; assisted in protecting a bunker
111. From a movie; possibly based on comics; 20s; The Legion of Night; sorcerer
113. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s;  recreation of man who beat his son
115. Original TV show character; 10s; gangster
119. Original movie character; 10s; scientist (probably geneticist)
123. Original TV show character; 10s; can move and shape water.
127. Original movie character; 00s; Air Force soldier
128. Original movie character; 10s; has control over electricity
131. Original TV show character; 10s; reporter
137. Original movie character; 10s; info sparse on this character and I don't recall her but appeared to have some involvement in event publicity
138. Original TV show character; 10s; attorney
139. Original TV show character; 20s; ; info sparse on this character and I don't recall him but appears to be on a subway train
142. Original movie character; 20s; mercenary
144. Original TV show character; 10s; tech company employee
145. From a commercial; based on comics; 00s; Tales of Suspense; seen in underground base
146. Original TV show character; 10s; helped try to capture major character
148. Original movie character; 20s; mystical creature
152. From a commercial; based on comics; 00s; Incredible Hulk; attempted to play volleyball
155. From a movie; based on comics; 20s; Amazing Spider-Man; doctor/scientist
156. Original TV show character; 10s; hospital administrator
157. Original TV show character; 10s; psychiatrist
159. Original TV show character; 10s; replaced by robotic being
160. Original TV show character; 10s; led crime family
163. From a movie; based on real person; 00s; seen at extreme sports event
164. Original TV show character; 10s; nurse
167. Original TV show character; 10s; helped in attempt to kill major villain
170. Original TV show character; 20s; film director
171. Original TV show character; 10s; forced to assist in experiements to increase major villain's power
175. Original movie character; 00s; some sort of captain
177. Original TV show character; 10s; police officer
178. From a commercial; based on comics; 00s; Fantastic Four; unlike other versions of this character, this version seems non-sentient
183. Original movie character; 00s; driver supplying a type of plant
184. Original movie character; 10s; former corrupt detective
187. From a commercial; based on comics; 00s; X-Men; seen in underground base
190. From a TV show; based on earlier character same continuity; 10s; artificial being who was part of a church
191. Original TV show character; 10s; police officer
192. Original TV show character; 10s; assigned the task of protecting super-powered person
193. Original TV show character; 10s; police officer
197. Original TV show character; 10s; artificial being/student
203. Original TV show character; 10s; criminal who lent out money
204. Original TV show character; 10s; executive producer
205. Original TV show character; 10s; chiurch member
206. Original TV show character; 10s; corrupt police officer
207. Original TV show character; 10s; attended house party
208. Original TV show character; 10s; baseball coach
210. Original TV show character; 10s; worked at energy comapny lab
211. From a movie; based on comics; 00s; Fantastic Four; landlady for boarding house
212. Original TV show character; 10s; struggled with luggage at bus depot
213. Original TV show character; 10s; government agent who investigated a murder
214. Original TV show character; 10s; worked inside barber shop
216. Original TV show character; 10s; police officer
218. Original movie character; 00s; agreed to help old lady move
220. Original TV show character; 10s; hated main character for perceived role in bother's death
221. Original TV show character; 10s; staff sergeant
222. Original TV show character; 10s; frequents Jamaican restaurant
223. Original TV show character; 10s; talent agent
226. Original movie character; 10s; part of conspiracy to deceive public
228. Original movie character; 10s; fan of particular superhero
230. Original movie character; 10s; enforcer
231. Original TV show character; 10s; priestess
232. Original TV show character; 10s; four-star general
234. Original TV show character; 10s; deputy chief of police
235. Original TV show character; 10s; police officer
236. Original TV show character; 10s; news company manager
241. Original TV show character; 10s; small café  owner
244. Original TV show character; 10s; rules planet
245. Original TV show character, though comics characters have same code name; 10s; music band fan
250. Original TV show character; 10s; has heterochromia
252. Original TV show character; 10s; unaware the charity org she works for is corrupt
254. Original TV show character; 10s; on list of people to be eliminated
255. From a movie; shares name with comics character but most likely a coincidence; 10s; general
256. Original movie character; 00s; negotiating agent
258. From a movie; based on comics; 10s; Journey Into Mystery; wife of a warrior
259. From a commercial; based on comics; 10s; Amazing Fantasy; encountered younger version of himself
260. Original TV show character; 10s; infected by parasitic ceature
261. Original TV show character; 10s; corrupt police detective
262. Original TV show character; 10s; assisted in capture of corrupt general
264. Original TV show character; 10s; active in 1931
265. From a commercial; based on comics; 10s; Captain America Comics; lashed with 280 while riding a motorcycle
271. Original TV show character; 10s; has artificial arm
272. Original TV show character; 00s; undercover familiar
273. Original movie character; 10s; former mechanic
275. Original TV show character; 10s; police officer
278. Original TV show character; 10s; air force general
279. From a movie; based on real person; 20s; criminal justice reporter
280. From a commercial; based on comics; 10s; Amazing Spider-Man; clashed with 265
281. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s; replaced major character's father
283. Original TV show character; 10s; attended some sort of committee session
287. Original TV show character; 10s; helped when a bunker was under siege
288. Original TV show character; 10s; traveling journalist
289. Original TV show character; 10s; ended relationship with boyfriend due to latter's drug addiction
290. Original TV show character; 10s; prostitute
292. From a commercial; based on mythology; 00s; norse mythology; his choice of weapon is key to identifying this version of the character
298. Original TV show character; 70s; wife of superhero
300. Original TV show character; 10s; police officer
303. Original TV show character; 10s; social club employee
304. Original TV show character; 10s; co-worker of main character
306. From a commercial; based on mythology; 00s; norse mythology; among heroes who considered their latest mission a wasted trip
307. Original TV show character; 70s; motocross driver
309. Original TV show character; 20s; part of family's flower shop
310. Original TV show character; 10s; helped ambush major character
311. Original TV show character; 70s;  blind martial arts master
312. Original TV show character; 10s; police officer
314. Original TV show character; 10s
319. Original TV show character; while there are multiple comics characters with he same name, that appears to be a coincidence; 10s
320. Original TV show character; 10s
322. Original TV show character; 10s
323. Original TV show character; 10s
325. Original TV show character; 20s
326. Original TV show character; 10s
329. Original TV show character; 10s
330. Original TV show character; 10s
332. Original movie character; 80s
335. Original TV show character; 10s
337. Original TV show character; 70s
341. Original TV show character; 10s
348. Original TV show character; 10s
349. Original movie character; 10s
350. From a movie; based on real person; 00s
351. Original TV show character; 20s
352. From a movie; based on comics; 10s
353. Original TV show character; 10s
355. Original TV show character; 10s
357. Original TV show character; 10s
360. Original TV show character; 10s
361. Original TV show character; 10s
364. Original TV show character; 10s
367. Original movie character; 10s
371. Original TV show character; 10s
372. Original movie character but likely named after real person; 10s
373. From a movie; based on comics; 10s; Peter Parker: Spider-Man
374. From a commercial; based on comics; 00s; Incredible Hulk
376. Original TV show character; 10s
377. From a movie; based on TV show; 00s; Spectacular Spider-Man
378. Original TV show character; 10s
379. Original TV show character; 10s
383. Original TV show character; 10s
388. Original TV show character; 10s
389. Original TV show character; 10s
390. From a TV show; based on comics; 10s; Daredevil
392. Original TV show character; 10s
393. Original TV show character; 10s
395. Original TV show character; 10s
396. Original TV show character; 10s
398. Original TV show character; 10s
400. Original movie character; 10s
403. Original TV show character; 10s
404. From a TV show; based on real person; 10s
405. Original TV show character ; 10s
407. Original movie character; 10s
409. Original TV show character; 10s
410. Original TV show character; 10s
412. Original TV show character; 10s

 

311 Li Sung, Hulk TV

298 Cookie (Hulk's wife), SNL "Superhero Party"

303 Eddie, Agent Carter

279 Dean Meminger, No Way Home

288 Griffin Sinclair, Jessica Jones

305 already identified

283 Chukwu, Agents of SHIELD

272 Zack Starr, Blade Series

260 Harold Simcoe, Agents of SHIELD