Both guesses correct; list updated.

404 Dapper Dan, Luke Cage

398 Wayne Olsen, Iron Fist

395 Hock, Helstrom

377 Bernard Houseman, Raimi Spider-Man

372 Conway, Amazing Spider-Man 2

351 Selim, Moon Knight

325 Nicolette, She-Hulk

323 Missy, Hawkeye

Cleaned up clues
12. Original movie character; 10s; member of criminal syndicate
13. Original TV show character; 10s; bank manager
15. Original movie character; 10s; hosted science videos
20. Original movie character; 00s; pulled gun on main character
28. Original TV show character; 10s; former member of terrorist group
29. Original TV show character; 10s; performed experiments on people
31. Original TV show character; 70s; reporter
33. Original TV show character; 10s; assisted in battle with artificial being
35. Original TV show character; 10s; student who attended lecture by major characters
40. Original TV show character; 10s; saved from rape attempt
43. Original movie character; 00s; medical doctor
44. Original TV show character; 10s; helped rescue major character while the latter was an infant
46. Original movie character; 00s; senator
50. Original movie character; 10s; high school teacher
51. Original TV show character; 10s; criminal figure
55. Original movie character; 10s; info sparse on this character and I don't recall them, but we only know her first name
56. From a commercial; based on comics; 00s; Captain America Comics; seen in underground base
59. Original TV show character; 10s; drug dealer
77. Original TV show character; 10s; embezzler
78. Original TV show character; 10s; has power of biological manipulation
79. Original TV show character; 20s; drove multiple major characters
84. Original TV show character; 10s; pilot
91. Original TV show character; 10s; police detective
101. Original TV show character; 10s; astronomer
108. Original TV show character; 10s; police captain
109. Original TV show character; 10s; assisted in protecting a bunker
113. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s;  recreation of man who beat his son
115. Original TV show character; 10s; gangster
119. Original movie character; 10s; scientist (probably geneticist)
123. Original TV show character; 10s; can move and shape water.
127. Original movie character; 00s; Air Force soldier
128. Original movie character; 10s; has control over electricity
131. Original TV show character; 10s; reporter
137. Original movie character; 10s; info sparse on this character and I don't recall her but appeared to have some involvement in event publicity
139. Original TV show character; 20s; info sparse on this character and I don't recall him but appears to be on a subway train
142. Original movie character; 20s; mercenary
144. Original TV show character; 10s; tech company employee
145. From a commercial; based on comics; 00s; Tales of Suspense; seen in underground base
146. Original TV show character; 10s; helped try to capture major character
148. Original movie character; 20s; mystical creature
152. From a commercial; based on comics; 00s; Incredible Hulk; attempted to play volleyball
159. Original TV show character; 10s; replaced by robotic being
163. From a movie; based on real person; 00s; seen at extreme sports event
164. Original TV show character; 10s; nurse
167. Original TV show character; 10s; helped in attempt to kill major villain
170. Original TV show character; 20s; film director
171. Original TV show character; 10s; forced to assist in experiements to increase major villain's power
175. Original movie character; 00s; some sort of captain
177. Original TV show character; 10s; police officer
178. From a commercial; based on comics; 00s; Fantastic Four; unlike other versions of this character, this version seems non-sentient
183. Original movie character; 00s; driver supplying a type of plant
184. Original movie character; 10s; former corrupt detective
187. From a commercial; based on comics; 00s; X-Men; seen in underground base
191. Original TV show character; 10s; police officer
192. Original TV show character; 10s; assigned the task of protecting super-powered person
193. Original TV show character; 10s; police officer
203. Original TV show character; 10s; criminal who lent out money
204. Original TV show character; 10s; executive producer
205. Original TV show character; 10s; chiurch member
206. Original TV show character; 10s; corrupt police officer
212. Original TV show character; 10s; struggled with luggage at bus depot
214. Original TV show character; 10s; worked inside barber shop
216. Original TV show character; 10s; police officer
218. Original movie character; 00s; agreed to help old lady move
219. Original TV show character; 10s; medical examiner
226. Original movie character; 10s; part of conspiracy to deceive public
228. Original movie character; 10s; fan of particular superhero
232. Original TV show character; 10s; four-star general
235. Original TV show character; 10s; police officer
250. Original TV show character; 10s; has heterochromia
252. Original TV show character; 10s; unaware the charity org she works for is corrupt
254. Original TV show character; 10s; on list of people to be eliminated
255. From a movie; shares name with comics character but most likely a coincidence; 10s; general
259. From a commercial; based on comics; 10s; Amazing Fantasy; encountered younger version of himself
261. Original TV show character; 10s; corrupt police detective
262. Original TV show character; 10s; assisted in capture of corrupt general
265. From a commercial; based on comics; 10s; Captain America Comics; lashed with 280 while riding a motorcycle
275. Original TV show character; 10s; police officer
280. From a commercial; based on comics; 10s; Amazing Spider-Man; clashed with 265
281. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s; replaced major character's father
287. Original TV show character; 10s; helped when a bunker was under siege
289. Original TV show character; 10s; ended relationship with boyfriend due to latter's drug addiction
292. From a commercial; based on mythology; 00s; norse mythology; his choice of weapon is key to identifying this version of the character
300. Original TV show character; 10s; police officer
304. Original TV show character; 10s; co-worker of main character
306. From a commercial; based on mythology; 00s; norse mythology; among heroes who considered their latest mission a wasted trip
310. Original TV show character; 10s; helped ambush major character
312. Original TV show character; 10s; police officer
314. Original TV show character; 10s; member of hate group
319. Original TV show character; while there are multiple comics characters with he same name, that appears to be a coincidence; 10s; gang leader
320. Original TV show character; 10s; brother of major character
322. Original TV show character; 10s; tribal leader
325. Original TV show character; 20s; pen pal (rule 23 applies to this character)
326. Original TV show character; 10s; crime family member
329. Original TV show character; 10s; non-US federal agent
330. Original TV show character; 10s; gang member
337. Original TV show character; 70s; scientist
341. Original TV show character; 10s; police detective
348. Original TV show character; 10s; created special mask
349. Original movie character; 10s; info sparse on this character and I don't recall him; only first name known
350. From a movie; based on real person; 00s; reporter
352. From a movie; based on comics; 10s; X-Men; father of major character
353. Original TV show character; 10s; auditioned for a role
355. Original TV show character; 10s; rapist
357. Original TV show character; 10s; police officer
360. Original TV show character; 10s; police sergeant
361. Original TV show character; 10s; hostage
364. Original TV show character; 10s; controlled lie deector sessions
367. Original movie character; 10s; assassin
374. From a commercial; based on comics; 00s; Incredible Hulk; among heroes who considered their latest mission a wasted trip
376. Original TV show character; 10s; professor of genetics under an alias
378. Original TV show character; 10s; army veteran
383. Original TV show character; 10s; co-worker of main character
392. Original TV show character; 10s; gang member
393. Original TV show character; 10s; therapist
396. Original TV show character; 10s; personal medical doctor
400. Original movie character; 10s; saved from burning car
407. Original movie character; 10s; medical doctor
409. Original TV show character; 10s; enforcer
410. Original TV show character; 10s; corrupt police officer
412. Original TV show character; 10s; criminal professor

 

325 is correct; list updated

20 Mrs Cesare, Punisher War Zone

137 Amy Page, Ghost Rider

163 Kenny Bartram, Fantastic Four (2005)

127 Ronald Coultrap, Iron Man