12 Oblo, GotG 2

Cleaned up clues:

13. Original TV show character; 10s; bank manager
28. Original TV show character; 10s; former member of terrorist group
29. Original TV show character; 10s; performed experiments on people
31. Original TV show character; 70s; reporter
33. Original TV show character; 10s; assisted in battle with artificial being
35. Original TV show character; 10s; student who attended lecture by major characters
40. Original TV show character; 10s; saved from rape attempt
44. Original TV show character; 10s; helped rescue major character while the latter was an infant
51. Original TV show character; 10s; criminal figure
56. From a commercial; based on comics; 00s; Captain America Comics; seen in underground base
59. Original TV show character; 10s; drug dealer
77. Original TV show character; 10s; embezzler
78. Original TV show character; 10s; has power of biological manipulation
84. Original TV show character; 10s; pilot
91. Original TV show character; 10s; police detective
101. Original TV show character; 10s; astronomer
108. Original TV show character; 10s; police captain
109. Original TV show character; 10s; assisted in protecting a bunker
113. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s;  recreation of man who beat his son
115. Original TV show character; 10s; gangster
123. Original TV show character; 10s; can move and shape water.
131. Original TV show character; 10s; reporter
144. Original TV show character; 10s; tech company employee
145. From a commercial; based on comics; 00s; Tales of Suspense; seen in underground base
146. Original TV show character; 10s; helped try to capture major character
152. From a commercial; based on comics; 00s; Incredible Hulk; attempted to play volleyball
159. Original TV show character; 10s; replaced by robotic being
164. Original TV show character; 10s; nurse
167. Original TV show character; 10s; helped in attempt to kill major villain
171. Original TV show character; 10s; forced to assist in experiements to increase major villain's power
177. Original TV show character; 10s; police officer
178. From a commercial; based on comics; 00s; Fantastic Four; unlike other versions of this character, this version seems non-sentient
184. Original movie character; 10s; former corrupt detective
187. From a commercial; based on comics; 00s; X-Men; seen in underground base
191. Original TV show character; 10s; police officer
192. Original TV show character; 10s; assigned the task of protecting super-powered person
193. Original TV show character; 10s; police officer
203. Original TV show character; 10s; criminal who lent out money
204. Original TV show character; 10s; executive producer
205. Original TV show character; 10s; chiurch member
206. Original TV show character; 10s; corrupt police officer
212. Original TV show character; 10s; struggled with luggage at bus depot
214. Original TV show character; 10s; worked inside barber shop
216. Original TV show character; 10s; police officer
219. Original TV show character; 10s; medical examiner
232. Original TV show character; 10s; four-star general
235. Original TV show character; 10s; police officer
250. Original TV show character; 10s; has heterochromia
252. Original TV show character; 10s; unaware the charity org she works for is corrupt
254. Original TV show character; 10s; on list of people to be eliminated
259. From a commercial; based on comics; 10s; Amazing Fantasy; encountered younger version of himself
261. Original TV show character; 10s; corrupt police detective
262. Original TV show character; 10s; assisted in capture of corrupt general
275. Original TV show character; 10s; police officer
281. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s; replaced major character's father
287. Original TV show character; 10s; helped when a bunker was under siege
289. Original TV show character; 10s; ended relationship with boyfriend due to latter's drug addiction
292. From a commercial; based on mythology; 00s; norse mythology; his choice of weapon is key to identifying this version of the character
300. Original TV show character; 10s; police officer
304. Original TV show character; 10s; co-worker of main character
306. From a commercial; based on mythology; 00s; norse mythology; among heroes who considered their latest mission a wasted trip
310. Original TV show character; 10s; helped ambush major character
312. Original TV show character; 10s; police officer
314. Original TV show character; 10s; member of hate group
319. Original TV show character; while there are multiple comics characters with he same name, that appears to be a coincidence; 10s; gang leader
320. Original TV show character; 10s; brother of major character
322. Original TV show character; 10s; tribal leader
326. Original TV show character; 10s; crime family member
329. Original TV show character; 10s; non-US federal agent
330. Original TV show character; 10s; gang member
341. Original TV show character; 10s; police detective
348. Original TV show character; 10s; created special mask
353. Original TV show character; 10s; auditioned for a role
355. Original TV show character; 10s; rapist
357. Original TV show character; 10s; police officer
360. Original TV show character; 10s; police sergeant
361. Original TV show character; 10s; hostage
364. Original TV show character; 10s; controlled lie deector sessions
374. From a commercial; based on comics; 00s; Incredible Hulk; among heroes who considered their latest mission a wasted trip
376. Original TV show character; 10s; professor of genetics under an alias
378. Original TV show character; 10s; army veteran
383. Original TV show character; 10s; co-worker of main character
392. Original TV show character; 10s; gang member
393. Original TV show character; 10s; therapist
396. Original TV show character; 10s; personal medical doctor
409. Original TV show character; 10s; enforcer
410. Original TV show character; 10s; corrupt police officer

Updated tally:
Agents of SHIELD    19
Jessica Jones    13
Daredevil TV    11
Runaways    7
Luke Cage    6
Cloak & Dagger    4
Gifted    4
Iron Fist    4
[non-soft drink beverage commercial]    3
Agent Carter    2
Punisher TV    2
[finance-related commercial]    2
Captain America: The Winter Soldier    1
[soft drink commercial]    1
[fast food related commercial]    1
Inhumans    1
Legion    1
[non-soft drink commercial with movie connection]    1
Incredible Hulk TV series    1
[toy commercial]    1
X-Men: First Class    1

 

254 Michael Lindon, Winter Soldier

13 Lillian, Punisher TV

205 Calvin, Runaways

261 Christian Blake, Daredevil TV

355 Reid Pearson, Jessica Jones

353 Britney Kovac, Jessica Jones

393 Levi van Kempen, Agents of SHIELD

Those are correct; list updated.

Expanded first 40 clues (to 232); will retally as at least one movie's missing from the tally

28. Original TV show character; 10s; former member of terrorist group; appeared in the third season of a series
29. Original TV show character; 10s; performed experiments on people for a financial corporation
31. Original TV show character; 70s; reporter; planted false evidence
33. Original TV show character; 10s; assisted in battle with artificial being as she attacked a base
35. Original TV show character; 10s; student at a special academy who attended lecture by major characters
44. Original TV show character; 10s; helped rescue major character from a village massacre while the latter was an infant
51. Original TV show character; 10s; criminal figure; ambushed by compromised FBI agents
56. From a commercial; based on comics; 00s; Captain America Comics; seen in underground base accessed from outside a building
59. Original TV show character; 10s; drug dealer  who dealt out of his apartment he shared with his grandmother
77. Original TV show character; 10s; embezzler whose wife left him
78. Original TV show character; 10s; has power of biological manipulation; asset for major villain
84. Original TV show character; 10s; pilot who crash landed a special jet
91. Original TV show character; 10s; police detective who attended junkyard crime scene
101. Original TV show character; 10s; astronomer; adoptive father of major character
108. Original TV show character; 10s; police captain in charge of correctional facility
109. Original TV show character; 10s; assisted in protecting a bunker from major villain who killed him
113. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s;  recreation of man who beat his son; real version is grandfather of major character
115. Original TV show character; 10s; gangster; asked by police to mediate gang meeting
123. Original TV show character; 10s; can move and shape water; part of underground movement
131. Original TV show character; 10s; reporter who interviewed major villain
144. Original TV show character; 10s; tech company employee; gave presentation
145. From a commercial; based on comics; 00s; Tales of Suspense; seen in underground base accessed from outside a building
146. Original TV show character; 10s; helped try to capture major character; member of some sort of clan
152. From a commercial; based on comics; 00s; Incredible Hulk; attempted to play volleyball but damaged the ball
159. Original TV show character; 10s; replaced by robotic being; some sort of agent
167. Original TV show character; 10s; helped army veteran in attempt to kill major villain;
171. Original TV show character; 10s; forced by mind control to assist in experiements to increase major villain's power
177. Original TV show character; 10s; police officer; forced by mind control to hold co-workjers at gunpoint
178. From a commercial; based on comics; 00s; Fantastic Four; unlike other versions of this character, this version seems non-sentient; "assisted" in team's fight with arch-foe
187. From a commercial; based on comics; 00s; X-Men; seen in underground base accessed from outside a building
191. Original TV show character; 10s; police officer; tried unsuccessfully to prevent a murder and a kidnapping
192. Original TV show character; 10s; assigned the task of protecting super-powered person but that person turned on him and killed him
193. Original TV show character; 10s; police officer; investigated whether a major character had been murdered
203. Original TV show character; 10s; criminal who lent out money for nightclub but demanded the money be returned after the club hasn't opened
204. Original TV show character; 10s; executive producer who eventually cast major character as a show's main character
212. Original TV show character; 10s; struggled with luggage at bus depot but was assisted by major villain
214. Original TV show character; 10s; worked inside barber shop; attempted to rob crime family
216. Original TV show character; 10s; police officer; visited medical centre afdter a shooting occurred there
219. Original TV show character; 10s; medical examiner; ordered to cover up an incident
232. Original TV show character; 10s; four-star general; served as the Chairman of the Joint Chiefs of Staff​
235. Original TV show character; 10s; police officer
252. Original TV show character; 10s; unaware the charity org she works for is corrupt
259. From a commercial; based on comics; 10s; Amazing Fantasy; encountered younger version of himself
262. Original TV show character; 10s; assisted in capture of corrupt general
275. Original TV show character; 10s; police officer
281. From a TV show; based on earlier character same continuity who was mentioned but not seen; 10s; replaced major character's father
287. Original TV show character; 10s; helped when a bunker was under siege
289. Original TV show character; 10s; ended relationship with boyfriend due to latter's drug addiction
292. From a commercial; based on mythology; 00s; norse mythology; his choice of weapon is key to identifying this version of the character
300. Original TV show character; 10s; police officer
304. Original TV show character; 10s; co-worker of main character
306. From a commercial; based on mythology; 00s; norse mythology; among heroes who considered their latest mission a wasted trip
310. Original TV show character; 10s; helped ambush major character
312. Original TV show character; 10s; police officer
319. Original TV show character; while there are multiple comics characters with he same name, that appears to be a coincidence; 10s; gang leader
320. Original TV show character; 10s; brother of major character
322. Original TV show character; 10s; tribal leader
326. Original TV show character; 10s; crime family member
329. Original TV show character; 10s; non-US federal agent
330. Original TV show character; 10s; gang member
341. Original TV show character; 10s; police detective
348. Original TV show character; 10s; created special mask
357. Original TV show character; 10s; police officer
360. Original TV show character; 10s; police sergeant
361. Original TV show character; 10s; hostage
374. From a commercial; based on comics; 00s; Incredible Hulk; among heroes who considered their latest mission a wasted trip
378. Original TV show character; 10s; army veteran
383. Original TV show character; 10s; co-worker of main character
392. Original TV show character; 10s; gang member
396. Original TV show character; 10s; personal medical doctor
409. Original TV show character; 10s; enforcer
410. Original TV show character; 10s; corrupt police officer

 

378 Lewis Wilson, Punisher TV

33 Navarro, Agents of SHIELD

51 Sophia Carter, Daredevil TV

91 Mark Bailey, Luke Cafe