During slower period (over a day with no guesses) I'll start adding ten clues starting from the chronologically earliest. Here are the ten plus the clues already known:

1 is an original TV character
3 is also an original TV character
4 is from a movie
6 is a TV character and part of a team
8 is from a movie
9 is an original TV character
10 is also an original TV character
11 is from TV
12 is an original TV character
13 is from a movie
While four of the TV characters above are from the same continuity, none of the above characters are from the exact show or movie.
89 despite a strong resemblance isn't the Ra's al Ghul from Young Justice specifically.
161 isn't Granny Goodness but she has a strong connection to her
228 isn't Blackfire

At least two characters from a TV continuity only appeared in a movie tie-in (the two characters I'm thinking of are not from the same continuity.) One of them, Dracula from Batman vs Dracula (The Batman continuity) has been found, the other hasn't.

Need to remove 24 - already identified.

68 Doctor Polaris, Brave and Bold

91 Hyde, Static Shock

180 Georgia Sivana, Kids Power Hour

Those are correct. List updated. For some reason some deleted images from the last batch got restored. Hopefully fixed now.

To make it easier for everyone to follow along and play, each time I post in this thread I'll bring the clues forward:
1 is an original TV character
3 is also an original TV character
4 is from a movie
6 is a TV character and part of a team
8 is from a movie
9 is an original TV character
10 is also an original TV character
11 is from TV
12 is an original TV character
While four of the TV characters above are from the same continuity, none of the above characters are from the exact show or movie.
89 despite a strong resemblance isn't the Ra's al Ghul from Young Justice specifically.
161 isn't Granny Goodness but she has a strong connection to her
228 isn't Blackfire
At least two characters from a TV continuity only appeared in a movie tie-in (the two characters I'm thinking of are not from the same continuity.) One of them, Dracula from Batman vs Dracula (The Batman continuity) has been found, the other hasn't.

I miswrote an earlier clue: as originally noted, despite a strong resemblance, 89 is not the Vandal Savage of Young Justice. Because that was partly my mistake in the summary, if you don't get to other guesses today (because you're allowed to replace the four incorrect answers), you can make a 31st guess next time. 72 is not from Kid Super Power Hour. 145 is not Mister Mind (notice he's using an ability that Mister Mind does not have). 181 is not from Justice League Unlimited. The rest are correct. List updated. For easy scanning I've also folded the above into the clues below.

1 is an original TV character
3 is also an original TV character
4 is from a movie
6 is a TV character and part of a team
9 is an original TV character
10 is also an original TV character
11 is from TV
12 is an original TV character
While four of the TV characters above are from the same continuity, none of the above characters are from the exact show or movie.
72 is not from Kid Super Power Hour
89 despite a strong resemblance isn't the Vandal Savage from Young Justice specifically.
145 is not Mister Mind
181 is not from Justice League Unlimited thus not DCAU
228 isn't Blackfire
At least two characters from a TV continuity only appeared in a movie tie-in (the two characters I'm thinking of are not from the same continuity.) One of them, Dracula from Batman vs Dracula (The Batman continuity) has been found, the other hasn't.

93 Stompa, Superman/Batman Apocalypse

272 Gentleman Ghost, Super Friends

249 Gentleman Ghost, Brave and Bold

283 Stompa, Brave and Bold

282 Kalibak, Superman TAS

136 Professor Pyg, Suicide Squad Hell to Pay

229 DeSaad, Super Friends

168 Calculator, Brave and Bold

227 Yo-Yo, during Flashpoint Paradox