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18 Myglom, Green Lantern
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25 Vinnie "the Shark" Starkey, BTAS
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134 Grant Walker, BTAS
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306 Puff, Static Shock
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38 Aquaramia, Static Shock
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30 Off-World Outlaw, Teen Titans
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40 Flamingo, Brave and Bold
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33 King Cobra, Brave and Bold
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47 Repeller, Batman Beyond
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43 Mastermind, Filmation (Superman/Aquaman)
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48 Skinman, Swamp Thing
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Those are all correct. List updated. In the case of 48, the clue referred to him working for Anton Arcane.
20 is an original TV character
29 is an original TV character
41 is an original TV character
50 is from a TV series; based on comics
53 is from a TV series; based on comics
55 is an original TV character who works for an existing comics character
57 is from a movie; based on a comics character but the comics character, while an adversary of sorts to a hero isn't really a villain
58 is from a TV series; based on comics
60 is an original TV character
65 is from a TV series; based on comics
66 is an original TV character
74 is from a movie; based on comics
77 is an original TV character
78 is an original TV character
80 is from a movie; based on comics
82 is an original TV character
87 is from a movie; based on comics
89 despite a strong resemblance to the Young Justice version isn't the Vandal Savage from any show; from a movie
145 is not Mister Mind
200 is not Boss/Bruno/Ugly Mannheim
264 isn't Man-Bat
267 is not from Crisis on Two Earths
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152 Anti-Monitor, Green Lantern
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206 Willie Watt, Batman Beyond
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130 Zander (leader of Kobra), Batman Beyond
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Those three are correct. List updated
20 is an original TV character
29 is an original TV character
41 is an original TV character
50 is from a TV series; based on comics
53 is from a TV series; based on comics
55 is an original TV character who works for an existing comics character
57 is from a movie; based on a comics character but the comics character, while an adversary of sorts to a hero isn't really a villain
58 is from a TV series; based on comics
60 is an original TV character
65 is from a TV series; based on comics
66 is an original TV character
74 is from a movie; based on comics
77 is an original TV character
78 is an original TV character
80 is from a movie; based on comics
82 is an original TV character
87 is from a movie; based on comics
89 despite a strong resemblance to the Young Justice version isn't the Vandal Savage from any show; from a movie
145 is not Mister Mind
200 is not Boss/Bruno/Ugly Mannheim
264 is not Man-Bat
267 is not from Crisis on Two Earths
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41 Mojo, BTAS
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50 Plastique, JLU
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55 Maven BTAS
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60 Cybron, Ruby-Spears Superman
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66 Amy, 82 Trouble, Superman TAS
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80 Menagerie, Superman vs the Elite
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90 Killer Croc, Batman: Gotham Knight
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89 Vandal Savage, Young Justice
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89 is incorrect. Lonewolf36 also guessed that so not having seen either Young Justice or the actual source, I compared the images and the differences are subtle but there. See below for another clue on that one. The rest are correct. List updated.
20 is an original TV character
29 is an original TV character
53 is from a TV series; based on comics
57 is from a movie; based on a comics character but the comics character, while an adversary of sorts to a hero isn't really a villain
58 is from a TV series; based on comics
65 is from a TV series; based on comics
74 is from a movie; based on comics
77 is an original TV character
78 is an original TV character
87 is from a movie; based on comics
89 despite a strong resemblance to the Young Justice version isn't the Vandal Savage from any show; from a movie
145 is not Mister Mind
200 is not Boss/Bruno/Ugly Mannheim
264 is not Man-Bat
267 is not from Crisis on Two Earths
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89 Vandal Savage, Suicide Squad: Hell to Pay
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89 is correct. Very similar looking. There's more of a tip-off in the clothing than in the face. I hadn't intended that image to be a misdirect for people; it's just what came up in a Google search. List updated.
20 is an original TV character
29 is an original TV character
53 is from a TV series; based on comics
57 is from a movie; based on a comics character but the comics character, while an adversary of sorts to a hero isn't really a villain
58 is from a TV series; based on comics
65 is from a TV series; based on comics
74 is from a movie; based on comics
77 is an original TV character
78 is an original TV character
87 is from a movie; based on comics
145 is not Mister Mind
200 is not Boss/Bruno/Ugly Mannheim
264 is not Man-Bat
267 is not from Crisis on Two Earths
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58 Misfit, Brave and Bold
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57 Arthur Reeves, Batman: Mask of the Phantasm
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65 Thomas Blake (not Catman here) DCAU